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Changes in Sympathetic Preganglionic Neurons and Associated Glial Cells following Injury

Coulibaly, Aminata P.
http://rave.ohiolink.edu/etdc/view?acc_num=miami1281032308

Abstract Details

2010, Master of Science, Miami University, Zoology.
The goal of this study was to characterize changes in the intermediolateral cell column (IML) of the spinal cord at level T1, at several time points, following transection of the cervical sympathetic trunk (CST). At 7 days following transection, significant increase was observed in the number of TrkB-ir oligodendrocytes and Iba1-ir microglial. In addition, a change in the morphology of both GFAP-ir astrocytes and microglia was also perceived. Insinuating that retrograde factors from the injury site are being released from the injured neurons and activating the glial cells. However, none of these changes were observed at any of the other time points examined. Analysis of the neuronal population showed a decrease in the preganglionic marker choline acetyltransferase (ChAT), and an increase in the neuronal injury marker activating transcription factor (ATF)-3. TUNEL assay and caspase-3 immunohistochemistry showed no apoptotic neurons, implying no cell death occurred in the IML following axonal transection.
Lori G. Isaacson, PhD (Advisor)
Kathleen Killian, PhD (Committee Member)
Paul James, PhD (Committee Member)
69 p.
Biology; Biomedical Research; Cellular Biology; Molecular Biology
Choline acetyltransferase; chat; cervical sympathetic trunk; cst; preganglionic neurons; intermediolateral cell column; IML; spinal cord; activating transcription factor-3; ATF-3; distal axonal injury; transection; astrocytes; microglia

Recommended Citations

Citations

  • Coulibaly, A. P. (2010). Changes in Sympathetic Preganglionic Neurons and Associated Glial Cells following Injury [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281032308

    APA Style (7th edition)

  • Coulibaly, Aminata. Changes in Sympathetic Preganglionic Neurons and Associated Glial Cells following Injury. 2010. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1281032308.

    MLA Style (8th edition)

  • Coulibaly, Aminata. "Changes in Sympathetic Preganglionic Neurons and Associated Glial Cells following Injury." Master's thesis, Miami University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281032308

    Chicago Manual of Style (17th edition)

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© 2010, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.
Release 3.2.12