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Isolation and Characterization of Sphingolipids in Arabidopsis thaliana

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2003, Bachelor of Science, Miami University, College of Arts and Sciences - Chemistry.
Total lipids were extracted from A. thaliana plant tissue and differential hydrolysis was utilized to determine the presence of sphingolipids. Silicic acid chromatography was utilized to separate lipids based upon their polarity, and fatty acid methyl esters were prepared from isolated A. thaliana sphingolipids. Gas chromatography and mass spectrometry were used to analyze the fatty acid content of the isolated sphingolipids. It was determined that the A. thaliana sphingolipids contained 16:0 and 18:0 fatty acids. The isolation and characterization scheme employed in this study will be used to investigate the sphingolipid fatty acid content of A. thaliana in the future.
Brenda Blacklock (Advisor)

Recommended Citations

Citations

  • Mecoli, M. (2003). Isolation and Characterization of Sphingolipids in Arabidopsis thaliana [Undergraduate thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111148450

    APA Style (7th edition)

  • Mecoli, Marc. Isolation and Characterization of Sphingolipids in Arabidopsis thaliana. 2003. Miami University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111148450.

    MLA Style (8th edition)

  • Mecoli, Marc. "Isolation and Characterization of Sphingolipids in Arabidopsis thaliana." Undergraduate thesis, Miami University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111148450

    Chicago Manual of Style (17th edition)