Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
muhonors1177457176.pdf (503.04 KB)
ETD Abstract Container
Abstract Header
The Cloning and Expression of Mouse Na+/H+ Exchanger 10
Author Info
McAfee, Jessica Leigh
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177457176
Abstract Details
Year and Degree
2007, Bachelor of Arts, Miami University, College of Arts and Sciences - Zoology.
Abstract
The Na+/H+ Exchanger (NHE) is a trans-membrane antiporter that regulates the pH of the cell by allowing for the exchange of a H+ out of the cell as a Na+ falls back into the cell. NHE-regulated intracellular pH levels have been theorized to affect the motility of vertebrate sperm. NHE1 and NHE5 isoforms have been previously localized to sperm, although a novel NHE isoform (mNHE10) has recently been discovered and localized to mice sperm. While the sequenced homology of the protein categorizes it as an NHE, it has yet to be characterized as a functional antiporter. To characterize the mouse NHE10, its gene must first be cloned and expressed in a eukaryotic cell line. Then its proton-extruding function can be characterized through the method of “proton suicide”. Future studies may lead to the possibility of targeting the mNHE10 protein in future male infertility or contraceptive treatments.
Committee
Paul James (Advisor)
Pages
25 p.
Keywords
Na+/H+ Exchanger
;
NHE
;
NHE10
;
sperm motility
;
intracellular pH
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
McAfee, J. L. (2007).
The Cloning and Expression of Mouse Na+/H+ Exchanger 10
[Undergraduate thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177457176
APA Style (7th edition)
McAfee, Jessica.
The Cloning and Expression of Mouse Na+/H+ Exchanger 10.
2007. Miami University, Undergraduate thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177457176.
MLA Style (8th edition)
McAfee, Jessica. "The Cloning and Expression of Mouse Na+/H+ Exchanger 10." Undergraduate thesis, Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1177457176
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
muhonors1177457176
Download Count:
762
Copyright Info
© 2007, all rights reserved.
This open access ETD is published by Miami University Honors Theses and OhioLINK.