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Investigation of a relationship between the core PAT family proteins and their expression in adipose tissue from specific depots of three mouse models with varying levels of GH signaling

Kolbash, Stacy L
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187645900

Abstract Details

2007, Master of Science (MS), Ohio University, Nutrition Science (Health and Human Services).
A better understanding of the storage and release of neutral lipids from adipocytes is vital to understanding obesity and diabetes. Work in recent years has revealed a central role for perilipin, a lipid droplet surface protein, for optimal triacylglycerol storage and fatty acid release. Perilipin belongs to the PAT family of proteins, including perilipin, ADRP, and TIP47. Perilipin and ADRP are most closely related and are responsible for controlling lipase access to TAG. Growth hormone (GH) has repeatedly been shown to induce lipolysis and inhibit lipogenesis although the mechanism is not well understood. The potential relationship between GH and the PAT family has yet to be examined. To address this, we utilized two mouse lines with altered GH signaling: 1) GH receptor gene disrupted (GHR -/-) mice; and 2) mice expressing bovine GH (bGH). Perilipin, ADRP, and TIP47 mRNA expression was compared in three adipose depots (subcutaneous, epididymal and retroperitoneal) in GHR -/-, bGH, and wild type (WT) mice on a high fat (HF) or low fat (LF) diet using quantitative real time PCR. Results showed a significant decrease in perilipin mRNA in the subcutaneous and retroperitoneal depots of bGH mice on the LF diet compared to GHR -/- and WT mice. However, there was no significant difference in perilipin mRNA expression between the GHR -/- and WT mice in any of the fat depots. ADRP mRNA expression levels are more variable than perilipin mRNA levels with respect to genotype and diet treatment. This research may provide insight into the relationship between GH and the lipid packaging functions of the PAT family and may aid in discovering a prospective target to aid in the treatment of obesity.
Darlene Berryman (Advisor)
105 p.
Health Sciences, Nutrition
GH; perilipin; PAT family; ADRP; TIP47; adipose depots

Recommended Citations

Citations

  • Kolbash, S. L. (2007). Investigation of a relationship between the core PAT family proteins and their expression in adipose tissue from specific depots of three mouse models with varying levels of GH signaling [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187645900

    APA Style (7th edition)

  • Kolbash, Stacy. Investigation of a relationship between the core PAT family proteins and their expression in adipose tissue from specific depots of three mouse models with varying levels of GH signaling. 2007. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187645900.

    MLA Style (8th edition)

  • Kolbash, Stacy. "Investigation of a relationship between the core PAT family proteins and their expression in adipose tissue from specific depots of three mouse models with varying levels of GH signaling." Master's thesis, Ohio University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187645900

    Chicago Manual of Style (17th edition)

ohiou1187645900
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© 2007, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.