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Identification of Genes with Altered Gene Expression in the Adipose Tissue of Mouse Models of Varied Growth Hormone Signaling

Swaminathan, Svetha
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1206377586

Abstract Details

2008, Master of Science (MS), Ohio University, Nutrition Science (Health and Human Services).
Microarray analyses were performed comparing mRNA expression levels in subcutaneous adipose tissue between mice with disruption in the growth hormone (GH) receptor gene (GHR-/- mice) and littermate controls. The data revealed that 87 genes were significantly upregulated and 72 genes were significantly downregulated in GHR-/- mice versus littermate controls. Among the 159 altered genes, a subgroup of five genes was chosen for confirmation by a second mRNA quantification method, real time reverse transcriptase polymerase chain reaction (RT-RT PCR). RT-RT PCR studies confirmed the altered gene expression for only three genes, angiotensinogen (Agt), adiponutrin (Adpn) and angiopoietin-like protein 4 (Angptl4). To better understand the role of GH in adipose specific expression of these three genes (Adpn, Agt, Angptl4), RT-RT PCR analysis was expanded to include an additional genotype of altered GH function, the bovine GH transgenic mice, an additional diet treatment high-fat diet, as well as two other adipose depots, the epididymal and retroperitoneal adipose depots. This thesis discusses in detail all gene alterations observed in the microarray analyses and the RT-RT PCR analyses for Adpn, Agt, Angptl4 in adipose tissue.
Darlene E. Berryman, PhD (Advisor)
Karen Coschigano, PhD (Committee Member)
Diana Manchester, MS (Committee Member)
142 p.
Biology; Biomedical Research; Cellular Biology; Molecular Biology; Nutrition
growth hormone; adipose tissue; growth hormone receptor knockout mice; bovine growth hormone transgenic mice; microarrays; real-time reverse transcriptase PCR

Recommended Citations

Citations

  • Swaminathan, S. (2008). Identification of Genes with Altered Gene Expression in the Adipose Tissue of Mouse Models of Varied Growth Hormone Signaling [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1206377586

    APA Style (7th edition)

  • Swaminathan, Svetha. Identification of Genes with Altered Gene Expression in the Adipose Tissue of Mouse Models of Varied Growth Hormone Signaling. 2008. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1206377586.

    MLA Style (8th edition)

  • Swaminathan, Svetha. "Identification of Genes with Altered Gene Expression in the Adipose Tissue of Mouse Models of Varied Growth Hormone Signaling." Master's thesis, Ohio University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1206377586

    Chicago Manual of Style (17th edition)

ohiou1206377586
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© 2008, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.