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Effects of Growth Hormone on Circulating Resistin Levels in Mice

Vijeyta, Fnu
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1346947127

Abstract Details

2012, Master of Science (MS), Ohio University, Food and Nutrition Sciences (Health Sciences and Professions).
Adipose tissue, along with its traditional role in lipid storage, also exhibits an extensive endocrine function secreting a number of anti- and proinflammatory hormones collectively known as adipokines. With obesity, the balance shifts towards an increase in proinflammatory hormones. One such adipokine, resistin, is found to have a positive correlation with inflammation and with insulin resistance. The aim of this study was to investigate circulating resistin levels in three mouse lines with altered growth hormone (GH) action. Bovine growth hormone (bGH) mice are giant, lean with lower insulin sensitivity while growth hormone antagonist (GHA) mice and growth hormone receptor knockout (GHR-/-) mice are dwarf and obese with either normal or higher insulin sensitivity, respectively. Blood plasma samples from three male mice lines: bGH, GHA, GHR-/- along with their littermate wild type (WT) control groups were collected following an overnight fast. The bGH mice (n = 6) were compared to WT controls (n = 5) at the age of 29 weeks, while 8 weeks bGH mice (n = 6) were compared to WT controls (n = 6). The GHA mice (n = 11) were matched to WT controls (n = 11) at the ages of 14, 26, 52 and 72 weeks. The GHR-/- mice (n = 6) were compared to WT controls (n = 10) at the age of 24 weeks. Mouse resistin immunoassay kits were used to quantify the resistin levels in the above mentioned mice. Leptin, adiponectin, insulin and body fat composition data have been reported from these mice previously. The resistin levels were found to be significantly higher in GHA mice when compared to controls at all ages except 14 weeks. Resistin levels remain relatively constant at four age points in GHA mice. Additionally, GHR-/- mice also had significantly higher resistin levels at the age of 24 weeks compared to controls. No significant differences were found in bGH mice for resistin levels when compared to controls. Positive and significant correlations were found for circulating resistin and adiponectin, leptin, insulin and fat mass in male GHA mice while resistin had a negative and significant correlation with body weight. On the other hand resistin was found to have a significant positive correlation with adiponectin in male GHR-/- mice. No differences in resistin levels were found in male bGH mice. Since GH is a diabetogenic hormone, it can be concluded that GH causes insulin resistance independent of circulating resistin levels.
Darlene Berryman (Advisor)
144 p.
Endocrinology; Food Science; Nutrition
Resistin; growth hormone; transgenic mice;

Recommended Citations

Citations

  • Vijeyta, F. (2012). Effects of Growth Hormone on Circulating Resistin Levels in Mice [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1346947127

    APA Style (7th edition)

  • Vijeyta, Fnu. Effects of Growth Hormone on Circulating Resistin Levels in Mice. 2012. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1346947127.

    MLA Style (8th edition)

  • Vijeyta, Fnu. "Effects of Growth Hormone on Circulating Resistin Levels in Mice." Master's thesis, Ohio University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1346947127

    Chicago Manual of Style (17th edition)

ohiou1346947127
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© 2012, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.