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Effects of Probiotic and Prebiotic Supplementation in Turkey Poults on Intestinal Morphology and MUC2 Gene Expression

Loeffler, Stephanie
http://rave.ohiolink.edu/etdc/view?acc_num=osu1414668950

Abstract Details

2014, Master of Science, Ohio State University, Animal Sciences.
Ohio has a 158 million dollar turkey industry. Gut growth and development occurs first before the turkey can realize its full muscle producing potential. Alternative and adjunctive approaches to decreasing the use of feed grade antibiotics are becoming an important area of research due to increasing consumer and legislative concerns with antibiotic resistance. Probiotics or supplemental dietary commensal microbes is one such potential approach as they can colonize the intestine, particularly in young animals with a relatively naive intestine microbiome. Intestinal mucosa is made up of mucin glycoproteins that play a key role in preventing the attachment and colonization of pathogenic bacteria. These proteins are made up of a protein backbone that is coded for by the MUC gene family. At hatch, the turkey intestine is relatively aseptic and therefore vulnerable to bacterial colonization from both commensal and pathogenic microbes. In this study, we determined the expression of MUC2, which codes for a secretory gel-forming mucin that is predominantly found in the small and large intestine, from immediately post-hatch through day 11 of age in poults fed a conventional starter diet, the starter diet supplemented with two commercial probiotics (A and B), or the starter diet supplemented with a commercial prebiotic. This was done by comparing the MUC2 transcription levels to the transcription level of a housekeeping gene. Multiple potential housekeeping genes were investigated and one, RPS13, was found to be stably expressed across all ages and treatments in the turkey poult. The effects of the supplemented diets on intestinal development were also analyzed. While MUC2 transcription increased with age, there were no significant effects due to diet. The intestinal parameters of villus height, area and crypt depth were all increased with supplementation of probiotic B and the prebiotic.
Michael Lilburn (Advisor)
Macdonald Wick (Committee Member)
Joseph Ottobre (Committee Member)
51 p.
Animal Sciences
Poult; probiotics; mucin; intestinal morphology

Recommended Citations

Citations

  • Loeffler, S. (2014). Effects of Probiotic and Prebiotic Supplementation in Turkey Poults on Intestinal Morphology and MUC2 Gene Expression [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1414668950

    APA Style (7th edition)

  • Loeffler, Stephanie. Effects of Probiotic and Prebiotic Supplementation in Turkey Poults on Intestinal Morphology and MUC2 Gene Expression. 2014. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1414668950.

    MLA Style (8th edition)

  • Loeffler, Stephanie. "Effects of Probiotic and Prebiotic Supplementation in Turkey Poults on Intestinal Morphology and MUC2 Gene Expression." Master's thesis, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1414668950

    Chicago Manual of Style (17th edition)

osu1414668950
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This open access ETD is published by The Ohio State University and OhioLINK.