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METAGENOMIC ANALYSIS OF PERIODONTAL BACTERIA ASSOCIATED WITH GENERALIZED AGGRESSIVE PERIODONTITIS

Altabtbaei, Khaled

Abstract Details

2016, Master of Science, Ohio State University, Dentistry.
Background: Generalized aggressive periodontitis (GAP) is a microbial disease with distinct clinical profile to the more clinically prevalent chronic periodontitis. Until recently, the capability to study the microbiome of the disease has not been possible. Methods: 17 patients with GAP were enrolled. Patients were excluded if they had uncontrolled systemic disease, pregnancy, or had received antibiotic therapy in the last 3 months. Paper point samples were taken from the deep and shallow sulci of the affected patients. Genomic DNA was isolated, sheared, size selected and sequenced using Illumina. Sequences were filtered, and functionally annotated using the MG-RAST pipeline. The intra- and inter-group functional profiles were compared between the deep and shallow pockets, as well as with healthy individuals and with patients with chronic periodontitis (CP), using Wald test and FDR (DESeq2). Results: Statistical analysis revealed no significant differences in the functional diversity between the shallow and deep pockets in generalized aggressive periodontitis, thus establishing that globalized functional capacity in GAP. Deep sites in GAP have higher functional capability than healthy sites, especially in regards to chemotaxis and motility. Finally, the functional capability of generalized aggressive periodontitis is distinct from chronic periodontitis. Conclusion: Distinct functional differences are found between health and disease in generalized aggressive periodontitis. These pockets, despite looking clinically healthy, have the same functional capacity as the deeper pockets in those patients. Moreover, these functional capability are distinct from the disease state in chronic periodontitis.
Purnima Kumar (Advisor)
Pooja Maney (Committee Member)
John Walters (Committee Member)
67 p.

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Citations

  • Altabtbaei, K. (2016). METAGENOMIC ANALYSIS OF PERIODONTAL BACTERIA ASSOCIATED WITH GENERALIZED AGGRESSIVE PERIODONTITIS [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1466590877

    APA Style (7th edition)

  • Altabtbaei, Khaled. METAGENOMIC ANALYSIS OF PERIODONTAL BACTERIA ASSOCIATED WITH GENERALIZED AGGRESSIVE PERIODONTITIS. 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1466590877.

    MLA Style (8th edition)

  • Altabtbaei, Khaled. "METAGENOMIC ANALYSIS OF PERIODONTAL BACTERIA ASSOCIATED WITH GENERALIZED AGGRESSIVE PERIODONTITIS." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1466590877

    Chicago Manual of Style (17th edition)