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Saulsbery Master's thesis_final.pdf (1.31 MB)

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Early life stress effects on neuroimmune function in limbic brain regions and mood-related behavior in male and female Sprague-Dawley rats

Saulsbery, Angela I
http://rave.ohiolink.edu/etdc/view?acc_num=osu1565784922963171

Abstract Details

2019, Master of Arts, Ohio State University, Psychology.
Adverse Childhood Experiences (ACEs) are widespread in the general population and appear to increase vulnerability to adult neuropsychiatric disorders. The biological mechanisms that confer this increased risk are poorly understood. Changes in neuroimmune cell number, phenotype, and activity are observed with stress, depression- and anxiety-like behavior in rodents, and mood disorders; these cells may also program adult behavior following early life stress. In this study, male and female Sprague-Dawley rats were subjected to early deprivation stress or control handling for 4hrs./day from PD2-20. Brains from rats killed at PD21 were processed for counting of mast cells and qPCR analysis of mRNA expression related to microglial phagocytosis, histamine synthesis, and blood-brain barrier proteins in limbic regions. Adult rats were tested for anxiety-like, stress coping, and risk assessment behavior. Stress increased granulated and total mast cell count in the hippocampus, as well as hippocampal expression of genes related to microglial phagocytosis. Stress decreased hippocampal histidine decarboxylase mRNA expression, amygdala cybb mRNA expression, exploratory behavior in the open field test (in males only), risk assessment behaviors, closed arm entries in the elevated plus maze, and time immobile in the forced swim test. When controlling for experimental condition, histidine decarboxylase mRNA expression and phagocytic gene expression were significantly negatively correlated. These experiments suggest potential neuroimmune mechanisms that program maladaptive adult behaviors following early life stress.
Kathryn Lenz, Ph.D. (Advisor)
Tamar Gur, M.D./Ph.D. (Committee Member)
Benedetta Leuner, Ph.D. (Committee Member)
78 p.
Neurosciences; Psychobiology; Psychology
early life stress; brain-resident immune cells; mast cells; microglia; risk assessment behaviors; Sprague-Dawley rats; microglial phagocytosis; early deprivation stress; stress

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Citations

  • Saulsbery, A. I. (2019). Early life stress effects on neuroimmune function in limbic brain regions and mood-related behavior in male and female Sprague-Dawley rats [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1565784922963171

    APA Style (7th edition)

  • Saulsbery, Angela. Early life stress effects on neuroimmune function in limbic brain regions and mood-related behavior in male and female Sprague-Dawley rats. 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1565784922963171.

    MLA Style (8th edition)

  • Saulsbery, Angela. "Early life stress effects on neuroimmune function in limbic brain regions and mood-related behavior in male and female Sprague-Dawley rats." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1565784922963171

    Chicago Manual of Style (17th edition)

osu1565784922963171
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© 2019, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.