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Full text of this paper is not available in the ETD Center. Copies may be available for inter-library loan from University of Cincinnati or may be available for purchase from Proquest/UMI

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STEROID RECEPTOR ACTION IN THE HIPPOCAMPUS IN STRESS AND AGING

MURPHY, ERIN KATHLEEN
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1020952919

Abstract Details

2002, MS, University of Cincinnati, Medicine : Interdisciplinary (Medical Science Scholars, Neuroscience).
Stress and aging are modulators of steroid receptor action in the hippocampus of rodents and humans. The glucocorticoid receptor (GR) is highly expressed in the hippocampus and shows marked alterations in function during aging. In particular, we have demonstrated age-related deficits in GR translocation from the cytosol to the nucleus as well as deficits in GR binding to DNA in the nucleus. The purpose of this study was to determine if these age-related decreases are specific to the GR or if another hippocampally expressed steroid receptor sharing structural and functional characteristics with GR, such as the androgen receptor (AR), was also affected. Additionally we wanted to determine if these age-related deficits would persist following an acute stressor. Thus we investigated cytosolic and nuclear protein levels and DNA binding of the GR and AR in the hippocampus in 3, 15, and 30-month Fisher 344 x Brown Norway F1 hybrid rats following 30 minutes of restraint stress. Additionally, we also investigated protein levels of 3 essential chaperone proteins (HSP-90, HSP-70, HSC-70) that are responsible for the proper activation and translocation of these receptors. We found no age-related deficits in translocation of the AR or GR in the control or stressed condition. We also found no age-related deficits in DNA binding for either receptor in the control or stress condition. Additionally, no age-related deficits were found for HSP-90, HSC-70 or HSP-70 protein levels in the cytosol or nucleus. Thus, we conclude that age-related deficits are population specific and we have uncovered a population of animals that exhibit normal steroid receptor function at all ages. This may help to determine protective molecular mechanisms responsible for successful aging.
Dr. James P. Herman (Advisor)
1 p.
Biology, Neuroscience
glucocorticoid receptor; androgen receptor; hippocampus; Fischer 344 x Brown Norway F1 hybrid rats; aging

Recommended Citations

Citations

  • MURPHY, E. K. (2002). STEROID RECEPTOR ACTION IN THE HIPPOCAMPUS IN STRESS AND AGING [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1020952919

    APA Style (7th edition)

  • MURPHY, ERIN. STEROID RECEPTOR ACTION IN THE HIPPOCAMPUS IN STRESS AND AGING. 2002. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1020952919.

    MLA Style (8th edition)

  • MURPHY, ERIN. "STEROID RECEPTOR ACTION IN THE HIPPOCAMPUS IN STRESS AND AGING." Master's thesis, University of Cincinnati, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1020952919

    Chicago Manual of Style (17th edition)

ucin1020952919
© 2002, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.