Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


wright1339615527.pdf (3.13 MB)

ETD Abstract Container

Abstract Header

Light-Limited Access to Fructose Alters Metabolic Function and Adipose Tissue Catecholaminergic Activity in Mice

Rodwan, Naima Salem
http://rave.ohiolink.edu/etdc/view?acc_num=wright1339615527

Abstract Details

2012, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Consumption of high levels of fructose produces glucose intolerance, sympathetic nervous system activation, and renal dysfunction. A diet high in fructose may play a role in the worldwide epidemic of obesity and diabetes. The aim of this study was to test the hypothesis that time (light/dark) limited access to fructose influences body fat, metabolic function, and adipose tissue catecholaminergic activity in mice. Male C57BL/6 mice were given standard chow and assigned to one of three groups: Control (n=10, water 24h); FL (n=11, 10% fructose solution during 12h light period); and FD (n=10, 10% fructose solution during 12h dark period). Metabolic parameters measured were body fat (BF, Echo-MRI), adipocyte cell size, and glucose tolerance as well as plasma glucose, adiponectin, insulin, leptin, triglycerides, and cholesterol. Catecholamine levels were measured directly in white adipose tissue (WAT) using high performance liquid chromatography (HPLC) with electrochemical detection. Immunochemistry was used for examining tyrosine hydroxylase (TH) staining in WAT and brainstem. Mice given fructose during the light phase (inactive period for mice) showed evidence for a diabetic like symptoms with prominent changes in adipose tissue fat. Results showed: 1) enhanced increase in BF and increased size of adipocytes in FL; 2) increased plasma insulin and leptin in FL without changes in glucose or glucose tolerance; 3) no change in cholesterol or triglycerides; 4) measurable amounts of norepinephrine (NE), epinephrine (EPI) in WAT without difference among groups; 5) enhanced staining for TH in WAT and brainstem locus coeruleus. The changes in adiposity occurred even though caloric intake was not different among groups. In conclusion, results document that restriction of fructose access to the light period produced pathological effects on metabolic function along with catecholaminergic activation in WAT and brainstem. These data may have clinical implications since the timing of intake may be important in the control of adiposity and the development of metabolic syndrome.
Mariana Morris, PhD (Advisor)
James Lucot, PhD (Committee Member)
Roberta Pohlman, PhD (Committee Member)
Mariana Morris, PhD (Other)
56 p.
Pharmacology
Light-Limited Access; fat tissue; caticholamine

Recommended Citations

Citations

  • Rodwan, N. S. (2012). Light-Limited Access to Fructose Alters Metabolic Function and Adipose Tissue Catecholaminergic Activity in Mice [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1339615527

    APA Style (7th edition)

  • Rodwan, Naima. Light-Limited Access to Fructose Alters Metabolic Function and Adipose Tissue Catecholaminergic Activity in Mice. 2012. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1339615527.

    MLA Style (8th edition)

  • Rodwan, Naima. "Light-Limited Access to Fructose Alters Metabolic Function and Adipose Tissue Catecholaminergic Activity in Mice." Master's thesis, Wright State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=wright1339615527

    Chicago Manual of Style (17th edition)

wright1339615527
585
© 2012, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.