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Hima Thesis Pharmatox2020 Revised.pdf (2.22 MB)

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Characterization of proteins found in serum and sputum samples from ventilator associated pneumonia patients

Yenuga, Hima Priya
http://orcid.org/0000-0003-1889-6805
http://rave.ohiolink.edu/etdc/view?acc_num=wright1590233445157576

Abstract Details

2020, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Ventilator associated pneumonia (VAP) is the most common nosocomial infection leading to increased mortality in ICU patients. The main goal of this project was to evaluate and analyze a number of different biomarkers of disease that may be involved in the transition to pneumonia in patients on ventilators. Patients in this study were in the ICU, required greater than or equal to 48 hours of mechanical ventilation, and had a Clinical Pulmonary Infection Score greater than or equal to 6. For this study we recruited 16 subjects who were on ventilator for more than 48 hours, obtained serum and sputum from each that was frozen at -80C and stored until use. Six patients developed pneumonia (VAP) and the rest were considered Non-VAP. Our goal was to analyze cytokine levels, Platelet-activating factor acteylhydrolase (PAFAh), procalcitonin, vitamin D, and pentraxins, in the sputum as well as serum samples from these patients. PAFAh is an enzyme that inactivates platelet activating factor, which is involved in cytokine release. Pentraxin3 is an early marker of lung injury. Using the Bio-Rad BioPlex system, we analyzed 27 cytokines in sputum and serum. Of these, we found that 6 were significantly increased in the sputum of VAP patients, while only 1 was significantly increased in the serum from VAP patients. The PAFAh assay revealed no significant difference between VAP and non-VAP patient sputum or serum, though there was a trend for lower PAFAh in VAP patients compared to non-VAP. Analysis for Pentraxin3 indicated that it was present in both sputum and serum though there was no significant difference. Sequencing of fragmented peptides from the sputum also revealed a peptide from pneumonia bacteria. In conclusion, the results strongly suggest that a larger study is needed, in addition to cytokines, other enzymes and proteins need to be explored as potential markers for early diagnosis and potential new treatments of VAP.
David R. Cool, Ph.D. (Advisor)
Ravi P. Sahu, Ph.D. (Committee Member)
Jeffery B. Travers, M.D., Ph.D. (Committee Member)
88 p.
Pharmacology
Ventilator Associated Pneumonia; Proteins; Sputum samples; Serum samples; Extracellular Vesicles; Pentraxin 3; Platelet Activating Factor Acetylhydrolase; CRP; ACE1; Cytokines

Recommended Citations

Citations

  • Yenuga, H. P. (2020). Characterization of proteins found in serum and sputum samples from ventilator associated pneumonia patients [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1590233445157576

    APA Style (7th edition)

  • Yenuga, Hima Priya. Characterization of proteins found in serum and sputum samples from ventilator associated pneumonia patients. 2020. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1590233445157576.

    MLA Style (8th edition)

  • Yenuga, Hima Priya. "Characterization of proteins found in serum and sputum samples from ventilator associated pneumonia patients." Master's thesis, Wright State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1590233445157576

    Chicago Manual of Style (17th edition)

wright1590233445157576
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This open access ETD is published by Wright State University and OhioLINK.