Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ysu1329661984.pdf (7.75 MB)

ETD Abstract Container

Abstract Header

Domain specific over-expression of a peptide encoded by an I-band domain of the human TTN gene; the role of titin exons 248 – 250 in C2C12 myogenesis

McCann, Stephanie M.
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329661984

Abstract Details

2011, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
In 1996, a form of Rippling Muscle Disease (RMD) was characterized as a heterogenic autoimmune neuromuscular disease. The mechanics of the main symptom, rolling contractions along the length of a muscle induced by stretching or tapping, are not yet understood, however, studies have suggested that the gigantic skeletal muscle protein titin may play a potential role. We hypothesize that an overexpression of a specific immunogenic titin domain would have an effect on the natural processes of cell growth, proliferation, and differentiation of C2C12 Mus musculus myoblast cells. A fusion plasmid was constructed to produce a fusion protein incorporating the immunogenic titin domain attached to a green fluorescent protein. C2C12 cultures were then transfected with the fusion plasmid and induced into differentiation over a two week period. Morphology and fluorescent patterns were analyzed using light and fluorescent microscopy techniques. Protein samples taken at various time points throughout the differentiation process were analyzed using SDS-PAGE. Techniques of the analysis of in vivo production of the immunogenic titin domain were developed. This preliminary data suggests that over-expression of an immunogenic titin domain has an effect on normal growth and differentiation of C2C12 cells. We have also shown that this method of study can be used to identify roles of not only this but other domains of the titin protein.
Gary Walker, PhD (Advisor)
David Asch, PhD (Committee Member)
Jonathan Caguiat, PhD (Committee Member)
118 p.
Cellular Biology; Molecular Biology
titin; autoimmune rippling muscle disease; C2C12 myogenesis; green fluorescent protein

Recommended Citations

Citations

  • McCann, S. M. (2011). Domain specific over-expression of a peptide encoded by an I-band domain of the human TTN gene; the role of titin exons 248 – 250 in C2C12 myogenesis [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329661984

    APA Style (7th edition)

  • McCann, Stephanie. Domain specific over-expression of a peptide encoded by an I-band domain of the human TTN gene; the role of titin exons 248 – 250 in C2C12 myogenesis. 2011. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329661984.

    MLA Style (8th edition)

  • McCann, Stephanie. "Domain specific over-expression of a peptide encoded by an I-band domain of the human TTN gene; the role of titin exons 248 – 250 in C2C12 myogenesis." Master's thesis, Youngstown State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329661984

    Chicago Manual of Style (17th edition)

ysu1329661984
592
© 2011, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.