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GADZEKPO FINAL 04 24 21 with cert.pdf (1.25 MB)

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Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus

Gadzekpo, Isaac Kwabena
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529

Abstract Details

2021, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Staphylococcus aureus is a notorious Gram positive bacterium that has been implicated in causing hospital-acquired and community-associated infections. The goal of this project was to retest previously selected S. aureus-specific phage clones from the phage library PhD-7 (New England BioLabs) and identify phage with a peptide that is able to bind to the capsular polysaccharide of S. aureus. The phage clones were amplified and PEG-purified prior to testing for specificity by performing ELISAs and sequencing. Whole cell bacterium ELISA using PEG-purified phage MMT5-2, 4, 5, 6 and 7 showed that all phage clones tested bound preferentially to S. aureus, except MMT5-7. Using purified carbohydrate as antigen, phage clone MMT5-4 showed high background but preferential binding to the capsule of S. aureus, while MMT5-6 showed non-specific binding. MMT5-2, 4, 5, 6 and 7 were selected for sequencing and MMT5-4 DNA sequence was translated into peptide sequence. The sequence revealed that the peptide displayed by the phage was SARXLLK and that the peptide was positively charged and hydrophobic in nature. Sequencing of our selected phage clone MMT5-4 agreed to Rao’s work with respect to the polar nature and hydrophobicity of the amino acid. However, our peptide did not show the LQX motif, as reported by Rao and coworkers. Nevertheless, it did show binding specificity against S. aureus as a whole cell bacterium, like Rao’s peptide and most importantly it showed specific binding to the capsule of S. aureus, type 5. This work is the first to report a peptide specific for S. aureus capsular carbohydrate. In future studies we hope to combine a toxin to the peptide to eradicate S. aureus infections.
Diana Fagan, PhD (Advisor)
Jonathan Caguiat, PhD (Committee Member)
David Asch, PhD (Committee Member)
63 p.
Biology
Staphylococcus aureus; Phage display; peptide

Recommended Citations

Citations

  • Gadzekpo, I. K. (2021). Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529

    APA Style (7th edition)

  • Gadzekpo, Isaac. Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus. 2021. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529.

    MLA Style (8th edition)

  • Gadzekpo, Isaac. "Selection of Phage Displaying Peptides Specific for Staphylococcus Aureus." Master's thesis, Youngstown State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619516455248529

    Chicago Manual of Style (17th edition)

ysu1619516455248529
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© 2021, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.
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