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Development of A New Methodology for Contact Angle Measurment on Monolayer of Cells

Jalalahmadi, Golnaz
http://rave.ohiolink.edu/etdc/view?acc_num=akron1374765701

Abstract Details

2013, Master of Science, University of Akron, Physics.
Surface wettability of living mammalian cells is important for many physiological processes including adhesion of bacterial and cancer cells to tissue surfaces. We employ contact angle measurements with polymeric aqueous two-phase systems (ATPS) to develop a method which will help to characterize wettability of cell layers. Living cells grown to a confluent monolayer are immersed in the lighter aqueous phase. A drop of the denser phase is dispensed to form a sessile drop on cells. The asymmetry of the drop due to roughness of the cells layer and the lack of reflection of the drop on the surface hinders using conventional drop shape methods to determine contact angles from the drop image. Thus, we develop a numerical module that employs a series of image processing techniques to extract the drop profile, fit polynomials, and apply statistical criteria to resolve contact angles. We demonstrate the utility of this approach to determine contact angles on cell monolayers.
Hossein Tavana, Dr. (Advisor)
Robert Mallik , Dr. (Advisor)
Alper Buldum, Dr. (Committee Member)
151 p.
Biophysics

Recommended Citations

Citations

  • Jalalahmadi, G. (2013). Development of A New Methodology for Contact Angle Measurment on Monolayer of Cells [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1374765701

    APA Style (7th edition)

  • Jalalahmadi, Golnaz. Development of A New Methodology for Contact Angle Measurment on Monolayer of Cells. 2013. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1374765701.

    MLA Style (8th edition)

  • Jalalahmadi, Golnaz. "Development of A New Methodology for Contact Angle Measurment on Monolayer of Cells." Master's thesis, University of Akron, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1374765701

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Akron and OhioLINK.