Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


YorkS.the (final comments 2).pdf (2.26 MB)

ETD Abstract Container

Abstract Header

Impact of Mechanically-Induced Microdamage and Gap Junctional Intercellular Communication on MLO-Y4 Viability and Sclerostin Expression

York, Spencer
http://rave.ohiolink.edu/etdc/view?acc_num=akron1415272760

Abstract Details

2014, Master of Science in Engineering, University of Akron, Biomedical Engineering.
Bone is continually undergoing remodeling, in which old bone is resorbed by osteoclasts and new bone is laid down by osteoblasts. This process provides a mechanism for bone to respond to changes in the mechanical environment. While the role of osteoblasts and osteoclasts in bone remodeling is clear, the cellular control of the process remains unknown. One theory is that osteocytes, found in the interior of bone, plays an important role in this process. Osteocytes may play a key role as they: possess physical processes, gap junctions, which allow communication between nearby osteocytes; they heavily express proteins integral to bone remodeling such as sclerostin; and, they are located inside of bone for detecting changes in mechanical load. In this work, MLO-Y4 cells, an osteocyte-like cell line, were cultured in vitro and exposed to mechanical load. Microdamage was induced in these cells using a mechanical loading platform and verified using lactate dehydrogenase activity. Further work with MLO-Y4 cells demonstrated that sclerostin activity increased at low surface strains, and that inhibition of gap junctional-mediated intercellular communication in mechanically-loaded osteocytes led to an increase in expression at higher strains, with a statistically significant difference from when communication was present. This suggested that osteocyte sclerostin expression was mediated by strain and gap junctional intercellular communication.
Marnie Saunders, Dr. (Advisor)
Hossein Tavana, Dr. (Committee Member)
Bi-min Newby, Dr. (Committee Member)
97 p.
Biomedical Engineering; Biomedical Research
Bone Mechanotransduction; Sclerostin; Gap Junctions

Recommended Citations

Citations

  • York, S. (2014). Impact of Mechanically-Induced Microdamage and Gap Junctional Intercellular Communication on MLO-Y4 Viability and Sclerostin Expression [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1415272760

    APA Style (7th edition)

  • York, Spencer. Impact of Mechanically-Induced Microdamage and Gap Junctional Intercellular Communication on MLO-Y4 Viability and Sclerostin Expression. 2014. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1415272760.

    MLA Style (8th edition)

  • York, Spencer. "Impact of Mechanically-Induced Microdamage and Gap Junctional Intercellular Communication on MLO-Y4 Viability and Sclerostin Expression." Master's thesis, University of Akron, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=akron1415272760

    Chicago Manual of Style (17th edition)

akron1415272760
517
© 2014, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.