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Exploring The Controlled Pellet Formation of Trichoderma reesei RUT-C30 for Improved Fermentation

Callow, Nicholas V.
http://orcid.org/0000-0002-1050-9738
http://rave.ohiolink.edu/etdc/view?acc_num=akron1427975356

Abstract Details

2015, Doctor of Philosophy, University of Akron, Chemical Engineering.
This work explored the unique influence of surfactants, shear, and fermentation conditions on the morphological and enzymatic production of Trichoderma reesei RUT-C30. Controlling fungal pellet formation and understanding of their behavior is critical to improving cellulase production. The inclusion of either Triton or rhamnolipid surfactant was found to induce pellet formation in T. reesei. While under the influence of a surfactant, the cellulase production increased. The number of pellets formed during growth was a function of the number of colony forming filamentous cells in the inoculum; pellets were only formed during periods of growth. Yet, the overall fungal amount was determined by the nutrient availability and not pellet concentration. The overall average pellet size was influenced by the fluid mixing conditions, with higher shear creating smaller pellets. This effect was replicated in both shake flasks and stir-tank fermentors, indicating similarity in fundamental mechanisms. Under phosphorus limiting conditions, fungal growth was successfully halted and continuous stationary phase cellulase production was achieved. Extended pellet based fermentations were possible by using nutrient limitation combined with surfactants. Nitrogen limitation was found to be ineffective for stationary phase cellulase production. An economic analysis, combined with Monte Carlo simulation of key uncertainties, indicated that the cellulase cost could be reduced when using both lower shear and nutrient control.
Lu-Kwang Ju, Dr. (Advisor)
Bi-min Zhang Newby, Dr. (Committee Member)
Nic Leipzig, Dr. (Committee Member)
Christopher Miller , Dr. (Committee Member)
Michael Konopka, Dr. (Committee Member)
237 p.
Chemical Engineering
Trichoderma reesei; cellulase; fermentation

Recommended Citations

Citations

  • Callow, N. V. (2015). Exploring The Controlled Pellet Formation of Trichoderma reesei RUT-C30 for Improved Fermentation [Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1427975356

    APA Style (7th edition)

  • Callow, Nicholas. Exploring The Controlled Pellet Formation of Trichoderma reesei RUT-C30 for Improved Fermentation. 2015. University of Akron, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1427975356.

    MLA Style (8th edition)

  • Callow, Nicholas. "Exploring The Controlled Pellet Formation of Trichoderma reesei RUT-C30 for Improved Fermentation." Doctoral dissertation, University of Akron, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1427975356

    Chicago Manual of Style (17th edition)

akron1427975356
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© 2015, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.