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Dissertation ShahM_final.pdf (4.65 MB)

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Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix

Shah, Mickey
http://orcid.org/0000-0001-6374-5091
http://rave.ohiolink.edu/etdc/view?acc_num=akron1542631193281779

Abstract Details

2018, Doctor of Philosophy, University of Akron, Integrated Bioscience.
Decellularized porcine myocardium has great potential in serving as a cardiac patch for heart repair after myocardial infarction (MI). However, using full thickness of decellularized porcine myocardium as a cardiac patch may lead to poor viability of the delivered cells due to perfusion limitation and may add an unwanted additional load to the infarcted heart. To address these issues we examined the feasibility of using a thin decellularized porcine myocardium slice (dPMS) as a cardiac patch to deliver therapeutic cells and assess whether this cardiac tissue engineering strategy would improve the function of injured myocardium caused by a MI. We started with obtaining thin dPMSs of various thicknesses by cryosectioning and then checked the effects of thickness on the mechanical properties of dPMS. Adipose derived Stem Cells (ASCs) from rats and pigs were isolated, characterized, and seeded on top of dPMSs with selected thicknesses. The viability, proliferation, infiltration, and differentiation of the seeded rat and pig ASCs were evaluated in vitro. The therapeutic outcomes of dPMS as a cardiac patch (with and without cells) were determined in vivo using a rat MI model. Our results confirmed that the fabricated dPMS can serve as a cell delivery platform to deliver a large amount of cells to injured myocardium with an increased cell retention rate as compared with direct cell injection. Furthermore, when used as a cardiac patch, dPMS integrates with host tissue, promotes host cell infiltration, increases vascularization in the infarcted area, and most importantly enhances cardiac function.
Ge Zhang, Dr. (Advisor)
Richard Londraville, Dr. (Committee Member)
Rouzbeh Amini, Dr. (Committee Member)
Liya Yin, Dr. (Committee Member)
Anand Ramamurthi, Dr. (Committee Member)
137 p.
Biomedical Engineering; Biomedical Research
Cardiac Tissue Engineering; Decellularized Extracellular Matrix; Adipose Derived Stem Cells-ASCs; Stem Cell Differentiation; Acute MI Mode; Cardiac Repair; Acellular Patch; Cell Delivery, Vascularization

Recommended Citations

Citations

  • Shah, M. (2018). Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix [Doctoral dissertation, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1542631193281779

    APA Style (7th edition)

  • Shah, Mickey. Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix. 2018. University of Akron, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1542631193281779.

    MLA Style (8th edition)

  • Shah, Mickey. "Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix." Doctoral dissertation, University of Akron, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1542631193281779

    Chicago Manual of Style (17th edition)

akron1542631193281779
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This open access ETD is published by University of Akron and OhioLINK.