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Optimizing Deposition of Matrix and Ionization Salt via Two-Step Sublimation in Sample Preparation for Surface-Layer Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging (SL-MALDI-TOF MSI)

Huang, Huan
http://rave.ohiolink.edu/etdc/view?acc_num=akron1619183035472425

Abstract Details

2021, Master of Science in Polymer Engineering, University of Akron, Polymer Science.
Studying the surface behavior of polymers is crucial to improve many applications of polymers or enlarge the range of applications. Surface Layer Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging (SL-MALDI-TOF MSI) is a novel technique based on the widely applied method of conventional MALDI mass spectrometry. Through SL-MALDI-TOF MSI, visualization of surfaces can be achieved. Moreover, this surface-specific technique can obtain the molecular composition of the surface layer, which we define as the layer within a few nanometers of the surface. Also, one can image the variation in this composition laterally across the surface. Previous research has proven that a two-step sublimation process of matrix and ionization salt is the best sample preparation for SL-MALDI MSI analysis. Nevertheless, the reproducibility and fidelity of the MALDI imaging still remain unsatisfying. From the preliminary investigations on the factors leading to nonuniform SL-MALDI-MSI signals from uniform film samples, it was concluded that the distributions of matrix and ionization salt in the chamber during sublimation are only two of the possible factors impacting uniformity, while thermal gradients were suspected to be a main factor causing the low fidelity. After eliminating thermal gradients from various aspects of the deposition process, the probability of obtaining a SL-MALDI image with high fidelity is greatly increased. However, the technique still suffers from low reproducibility and the occasional appearance of a secondary distribution in the mass spectra resulting from the salt clusters. A speculation is made suggesting that these limitations can be overcome potentially by using proper pretreatment for the ionization salt.
Mark Foster (Advisor)
Chrys Wesdemiotis (Committee Member)
78 p.
Analytical Chemistry; Polymers; Technology
Surface layer; MALDI MS imaging; Sublimation

Recommended Citations

Citations

  • Huang, H. (2021). Optimizing Deposition of Matrix and Ionization Salt via Two-Step Sublimation in Sample Preparation for Surface-Layer Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging (SL-MALDI-TOF MSI) [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1619183035472425

    APA Style (7th edition)

  • Huang, Huan. Optimizing Deposition of Matrix and Ionization Salt via Two-Step Sublimation in Sample Preparation for Surface-Layer Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging (SL-MALDI-TOF MSI) . 2021. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1619183035472425.

    MLA Style (8th edition)

  • Huang, Huan. "Optimizing Deposition of Matrix and Ionization Salt via Two-Step Sublimation in Sample Preparation for Surface-Layer Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging (SL-MALDI-TOF MSI) ." Master's thesis, University of Akron, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1619183035472425

    Chicago Manual of Style (17th edition)

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