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Isolation and Characterization of a Suspected Phytoalexin from Wilted Red Maple Leaves

Baisden, Jared T
http://rave.ohiolink.edu/etdc/view?acc_num=auhonors1367972336

Abstract Details

2013, Bachelor of Science, Ashland University, Biology/Toxicology.
Wilted red maple leaves are toxic to horses, causing death by oxidation of hemoglobin and inducing anemia. Gallic acid derivatives have been identified as the main oxidants present in the leaves. However, our work has found that a previously unknown phytoalexin is produced by wilting red maple leaves. Phytoalexins are defensive compounds produced by plants in response to fungal attack. These compounds often have a range of biological activities. The unidentified compound from red maple, which fluoresces blue in certain TLC systems, is present only after wilting. The objective of this study is to identify and characterize this compound so that its toxicity can be determined. Wilted leaves were collected, dried, and extracted with methanol. Leaf extracts have been purified through repeated thin layer chromatography and column chromatography. After successful purification, the structure of the compound will be confirmed by NMR and mass spectral analysis. This research will provide insight regarding the mechanism of fungal defense in Acer rubrum and may also be relevant to the known toxicity of wilted red maple leaves to horses.
Jeffrey Weidenhamer, Ph.D. (Advisor)
Robert Bergosh, Ph.D. (Other)
36 p.
Analytical Chemistry; Biochemistry; Biology; Botany; Chemistry
Phytoalexin; natural products isolation; Hemolytic Anemia in horses; phytochemistry; red maple

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Citations

  • Baisden, J. T. (2013). Isolation and Characterization of a Suspected Phytoalexin from Wilted Red Maple Leaves [Undergraduate thesis, Ashland University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=auhonors1367972336

    APA Style (7th edition)

  • Baisden, Jared. Isolation and Characterization of a Suspected Phytoalexin from Wilted Red Maple Leaves. 2013. Ashland University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=auhonors1367972336.

    MLA Style (8th edition)

  • Baisden, Jared. "Isolation and Characterization of a Suspected Phytoalexin from Wilted Red Maple Leaves." Undergraduate thesis, Ashland University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=auhonors1367972336

    Chicago Manual of Style (17th edition)

auhonors1367972336
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© 2013, all rights reserved.
This open access ETD is published by Ashland University Honors Theses and OhioLINK.