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Analysis and Characterization of Microbes from Ancient Glacial Ice

Veerapaneni, Ram S.
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1256565133

Abstract Details

2009, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
The objective of this research was to study the bacterial and fungal composition of icecores from Arctic and the Antarctic regions to understand their geographical and temporal distribution. Cosmopolitan microbes dominate these environments and have been deposited primarily by winds, birds, Ocean currents and mammals. Atmospheric currents play a significant role in the transport of these microorganisms to the polar regions. We hypothesize that the geographical isolation of the Antarctic region from other land masses, compared to the Arctic region, affects the transport of the microorganisms to the Antarctic region, resulting in lower number of microbes entrapped in the Antarctic ice. Four ice cores each were analysed from the Arctic (GISP2D core) and the Antarctic (Vostok 5G core) regions dating back to 10,000 YBP, 57,000 YBP, 105,000 YBP and 155,000 YBP using culturing, PCR, sequencing and SEM techniques. We report the isolation of fungi and bacteria from six of the eight ice cores analysed. Fungi most closely related to Rhodotorula, Penicillium, Cladosporium, Alternaria, Aspergillus and Cryptococcus, and bacteria related to Caulobacter and Bacillus were identified. The ice cores from Arctic had higher number of microorganisms and species richness than the core sections from the Antarctic region. Phylogenetic studies were done to compare the organisms with one another and also with the conspecifics. Some sequences showed high similarity to contemporary species, while some did not group closely with the present day organisms. Fungi isolated from the different locations, and closely related to the same genus, did not show high similarity with one another.
Scott Rogers (Advisor)
Kurt Panter (Committee Chair)
Carmen Fioravanti (Committee Member)
Vipaporn Phuntumart (Committee Member)
John Castello (Committee Member)
122 p.
Molecular Biology
GISP2D; Vostok; Fungi; Cold environments

Recommended Citations

Citations

  • Veerapaneni, R. S. (2009). Analysis and Characterization of Microbes from Ancient Glacial Ice [Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1256565133

    APA Style (7th edition)

  • Veerapaneni, Ram. Analysis and Characterization of Microbes from Ancient Glacial Ice. 2009. Bowling Green State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1256565133.

    MLA Style (8th edition)

  • Veerapaneni, Ram. "Analysis and Characterization of Microbes from Ancient Glacial Ice." Doctoral dissertation, Bowling Green State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1256565133

    Chicago Manual of Style (17th edition)

bgsu1256565133
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© 2009, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.