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Genetic engineering of S-layer of Caulobacter crescentus for bioremediation of heavy metals

Patel, Jigar J.
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1257191921

Abstract Details

2009, Master of Science (MS), Bowling Green State University, Biological Sciences.
The recombinant strain JS4022/p723-6H (Patel, et al., 2009), expressing RsaA-6His fusion protein was examined for its ability to sequester Cd(II) from the bacterial growth medium. When mixed with 1 ppm CdCl2, JS4022/p723-6H removed 94.3~99.9% of the Cd(II), whereas the control strain removed only 11.4~37.0%, depending on experimental conditions. The effective contact time of the cells and Cd(II) was as short as 15 min. When higher concentrations of CdCl2 were tested, JS4022/p723-6H consistently demonstrated enhanced binding capacity over the control strain. At 15 ppm of Cd(II), each gram of JS4022/p723-6H dry cells retrieved 16.0 mg of Cd(II), comparing to 11.6 mg/g achieved by the control strain. The morphology of the biofilms formed by Caulobacter crescentus recombinant strain JS4022/p723-6H, which expresses hexahistidine peptides anchored to its surface layer protein RsaA, was examined. The density of the biofilm reached a maximum after 48 h of incubation and was not affected by exposure to cadmium. When treated with 0.4 ppm Cd(II), biofilms formed by the engineered strain removed 76.9% of the total metal, whereas a control strain only removed 13.5%. This work provides a potential cost-effective solution toward bioremediation of heavy metals from aqueous systems, and effective means of constructing large scale remedial bioreactors in a cost effective manner.
Zhaohui Xu, PhD (Advisor)
Robert McKay, PhD (Committee Member)
Carol Heckman, PhD (Committee Member)
46 p.
Biology; Environmental Science
Caulobacter crescentus; S-layer; Immobilization; Biofilm; Cadmium; Heavy metals; Bioremediation

Recommended Citations

Citations

  • Patel, J. J. (2009). Genetic engineering of S-layer of Caulobacter crescentus for bioremediation of heavy metals [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1257191921

    APA Style (7th edition)

  • Patel, Jigar. Genetic engineering of S-layer of Caulobacter crescentus for bioremediation of heavy metals. 2009. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1257191921.

    MLA Style (8th edition)

  • Patel, Jigar. "Genetic engineering of S-layer of Caulobacter crescentus for bioremediation of heavy metals." Master's thesis, Bowling Green State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1257191921

    Chicago Manual of Style (17th edition)

bgsu1257191921
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© 2009, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.