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Microarray Analysis of Differential Expression of Genes in Shoot Apex and Young Leaf of English Ivy (Hedera helix L. cv. Goldheart)

Shin, Seung-Geuk
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1273946268

Abstract Details

2010, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
Shoot apical meristems (SAMs) of higher plants maintain a population of pluripotent cells which continue to divide throughout the life of plant and provide cells for development of all the above-ground organs after embryogenesis. Since the plant is sessile, maintenance of stem cells in the SAM and appropriate differentiation are crucial for the plants to adapt to the changing environments. Shoot apex of the plant is the very tip of the shoot, in which the SAM resides. To analyze the differential gene expression patterns between the shoot apex and the very young leaf, transcriptomes from the two tissue types of a variegated variety of English ivy (Hedera helix L. cv. Goldheart) plants, were hybridized on Arabidopsis thaliana cDNA microarrays, using cross-species hybridization (CSH). Among 11,255 cDNA probes excluding ‘BLANK’ and ‘bad’ spots, 2,597 features produced signals that were greater than background levels, which constitutes 23% of the total number of usable probes on the microarray. One hundred seventy four genes were expressed statistically differentially (fold change >=2 and p=0.05). Of these, 60 were in the shoot apex and 114 were in the young leaf. Functional categorization based on the genome/protein databases and a pathway analysis revealed some of the tissue-specific biological processes and identified some of the genes involved. The annotated and/or predicted roles of those genes in the tissue-specific biological processes were described and discussed in relation to the plant development.
Scott O. Rogers, PhD (Advisor)
Kit C. Chan, PhD (Committee Chair)
Carmen F. Fioravanti, PhD (Committee Member)
Helen Michaels, PhD (Committee Member)
Paul F. Morris, PhD (Committee Member)
161 p.
Bioinformatics
microarray analysis; cross-species hybridization; shoot apical meristem; shoot apex; Hedera helix Goldheart; variegated ivy; electronic Northerns; eNorthern; electronic fluorescent pictograph; eFP; GeneSpring; shoot development

Recommended Citations

Citations

  • Shin, S.-G. (2010). Microarray Analysis of Differential Expression of Genes in Shoot Apex and Young Leaf of English Ivy (Hedera helix L. cv. Goldheart) [Doctoral dissertation, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1273946268

    APA Style (7th edition)

  • Shin, Seung-Geuk. Microarray Analysis of Differential Expression of Genes in Shoot Apex and Young Leaf of English Ivy (Hedera helix L. cv. Goldheart). 2010. Bowling Green State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1273946268.

    MLA Style (8th edition)

  • Shin, Seung-Geuk. "Microarray Analysis of Differential Expression of Genes in Shoot Apex and Young Leaf of English Ivy (Hedera helix L. cv. Goldheart)." Doctoral dissertation, Bowling Green State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1273946268

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Bowling Green State University and OhioLINK.