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Oral Fluid Method Validation for Bowling Green State University

Bunch, Nathan
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1586969951770212

Abstract Details

2020, Master of Science (MS), Bowling Green State University, Forensic Science.
Oral fluid (OF) is rapidly becoming a new media for assisting law enforcement in determining if a subject is driving under the influence of drugs (DUID). Preliminary research shows that drugs can be identified in OF in conjunction with blood, and drug concentrations in OF and blood correlate. Despite availability of several roadside devices to test for drugs in OF, the roadside devices are considered a presumptive test. The results from these roadside tests must be confirmed with a validated liquid chromatography- mass spectrometer (LC-MS) instrumentation. A validated instrument for confirmation of OF results is important, and this study validates BGSU’s Shimadzu 8050 LC-MS. For validation, the instrument must pass various guidelines set by Scientific Working Group for Forensic Toxicology (SWGTOX) Standard Practices for Method Validation in Forensic Toxicology and others for accuracy, precision, linearity, Limit of Detection (LOD) and Limit of Quantitation (LOQ), carryover, interference, stability, and matrix effects. Due to the Covid-19 global pandemic, only accuracy, precision, linearity, and LOD and LOQ were accessed. The validation studies were conducted over five days (not consecutive) with two runs being conducted during each 24-hour period for a total of 10 runs. A total of 83 different analytes were accessed. The 83 analytes covered a broad range of drugs with abuse potential. The results of the validation study showed that the instrument is highly precise for the vast majority of analytes, but the cannabinoids, particularly delta-9-tetrahydrocannabinol (THC), were troublesome. Linearity for all analytes were accessed using the R^2 of the calibration curve, and all analytes were above the 0.95 limit. The LOD and LOQ study proved that the cutoff for each analyte is correctly higher than the factor of 2 limit for cutoff/LOQ.
Jon Sprague, RPh, PhD (Advisor)
Travis Worst, PhD (Committee Member)
Phillip Gibbs, PhD (Committee Member)
137 p.
Biology; Chemistry; Molecular Chemistry; Organic Chemistry; Pharmaceuticals; Pharmacology; Pharmacy Sciences; Physical Chemistry; Statistics; Toxicology
Forensic; Science; Bowling; Green; State; University; BGSU; Chemistry; Drugs; Oral; Fluid; Blood; Saliva; Validation; Study; Precision; Accuracy; Liquid Chromatography; LC; Mass Spectrometry; MS; LC-MS; LC-MSMS; method; validation; thc; roadside; testing

Recommended Citations

Citations

  • Bunch, N. (2020). Oral Fluid Method Validation for Bowling Green State University [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1586969951770212

    APA Style (7th edition)

  • Bunch, Nathan. Oral Fluid Method Validation for Bowling Green State University. 2020. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1586969951770212.

    MLA Style (8th edition)

  • Bunch, Nathan. "Oral Fluid Method Validation for Bowling Green State University." Master's thesis, Bowling Green State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1586969951770212

    Chicago Manual of Style (17th edition)

bgsu1586969951770212
488
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This open access ETD is published by Bowling Green State University and OhioLINK.