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Sarah Wagner Thesis 4_4_21.pdf (1.57 MB)

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Efficiency of DNA Recovery from Different Swab Types by qPCR

Wagner, Sarah Jean
http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1616962034143618

Abstract Details

2021, Master of Science (MS), Bowling Green State University, Forensic Science.
Microbial genetics is an emerging area of study within the field of forensic science. Topics of research include bioterrorism investigation and the relevance of microbes in human identification. In the area of forensic DNA analysis, a developing method includes the supplementation of human short tandem repeat profiles with microbiome profiles; however, studies concerning the collection of microbial DNA in a forensic context are scarce. Thus, the primary aim of this study was to evaluate the relative yield of microbial DNA from cotton and flocked swabs, both common tools in the discipline of forensic biology. To pursue this aim, the relative DNA yield from Proteus mirabilis samples pipetted onto the swabs was compared via qPCR. Compared to the DNA recovery of the non-manipulated positive control, Copan FLOQSwabs and Puritan cotton swabs yielded 17.52 % ± 2.47% (mean ± standard error) and 61.13% ± 13.39%, respectively. Furthermore, the manipulation control group, which demonstrated the effects of vortexing and centrifugation on DNA yield, resulted in a 61.56% ± 8.02% recovery compared to the positive control. The DNA recovery from the cotton swabs was not significantly different than the DNA recovery for the manipulation controls, but the recovery of microbial DNA using the flocked swabs was reduced significantly as compared to either the cotton swabs or the manipulation controls. Such results illustrate a greater DNA recovery ability of cotton swabs relative to Copan FLOQSwabs. However, further studies regarding the relative collection abilities of these swabs are required to fully assess their performance under field conditions.
Vipaporn Phuntumart, PhD (Advisor)
Travis Worst, PhD (Committee Member)
Crystal Oechsle, PhD (Committee Member)
62 p.
Biology; Genetics; Microbiology
forensic science; microbiology; DNA; qPCR; swabs

Recommended Citations

Citations

  • Wagner, S. J. (2021). Efficiency of DNA Recovery from Different Swab Types by qPCR [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1616962034143618

    APA Style (7th edition)

  • Wagner, Sarah. Efficiency of DNA Recovery from Different Swab Types by qPCR. 2021. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1616962034143618.

    MLA Style (8th edition)

  • Wagner, Sarah. "Efficiency of DNA Recovery from Different Swab Types by qPCR." Master's thesis, Bowling Green State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1616962034143618

    Chicago Manual of Style (17th edition)

bgsu1616962034143618
283
© 2021, all rights reserved.
This open access ETD is published by Bowling Green State University and OhioLINK.