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Characterization of the osteogenic cell lineage

Bruder, Scott Philip
http://rave.ohiolink.edu/etdc/view?acc_num=case1054845574

Abstract Details

1990, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
Studies of the origin, commitment, and differentiation of osteogenic cells can be facilitated by cell-specific markers. To this end, monoclonal antibodies against surface determinants on osteogenic cells were generated by immunizing naive mice with a heterogeneous population of chick embryonic bone cells. Four monoclonal cell lines, SB-1, SB-2, SB-3, and SB-5, which each secrete a different antibody against the surface of osteogenic cells, were immortalized and subsequently used to study the developmental progression of osteogenic cells in a variety of developmental systems. Detailed morphologic analyses during the process of bone formation in embryonic chick tibiae, organ culture of embryonic chick periosteum, and in vivo diffusion chamber culture of avian marrow stem cells reveal that a precise sequence of cell surface alterations occur during osteogenic differentiation. Of particular interest is the observation that this sequence of cellular events is conserved in all the developmental systems studied. Results of these analyses indicate the Pre-Osteoblastic cells, which are recognized by antibody SB-1, proceed through their lineage to become Transitory 1 Osteoblasts, which are immunostained by SB-1 and SB-3. These cells continue differentiating to become Transitory 2 Osteoblasts, which are reactive wit h antibodies SB-1, SB-3, and SB-2. These non-secretory cells then elaborate a type I collagen-rich osteoid matrix as SB-1, SB-3, and SB-2-positive Secretory Osteoblasts. Those Secretory Osteoblasts which are incorporated into the bone matrix then loose surface antigens SB-1 and SB-3, while they begin expressing the SB-5 antigen on their surface. The resulting Osteocytic Osteoblasts, which are reactive with both the SB-2 and SB-5 antibodies, finally undergo a terminal differentiation step to become Osteocytes. This final lineage step is characterized by the loss of the SB-2 antigen and the retention of SB-5 immunoreactivity. Additional studies have focused on characterizing the identity of the antigens recognized by these antibodies. Interestingly, SB-1 is shown to react with alkaline phosphatase isoenzymes present in bone, liver, kidney, cartilage and intestine. While the alkaline phosphatase isoform in embryonic bone has an apparent molecular weight of 155 kD, the intestinal isoenzyme is approximately 185 kD. In both isoforms, however, the immunoreactive epitope is stable to SDS denaturation, not associated with the active site of the enzyme, and dependent on disulfide bonds which impart secondary structure to the protein.
Arnold Caplan (Advisor)
317 p.
osteogenic cell lineage

Recommended Citations

Citations

  • Bruder, S. P. (1990). Characterization of the osteogenic cell lineage [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1054845574

    APA Style (7th edition)

  • Bruder, Scott. Characterization of the osteogenic cell lineage. 1990. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1054845574.

    MLA Style (8th edition)

  • Bruder, Scott. "Characterization of the osteogenic cell lineage." Doctoral dissertation, Case Western Reserve University, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054845574

    Chicago Manual of Style (17th edition)

case1054845574
569
© 1990, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.