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Isolation and characterization of hormone-autonomous tumors of Arabidopsis thaliana

Persinger, Sharon Marie
http://rave.ohiolink.edu/etdc/view?acc_num=case1055865093

Abstract Details

1991, Doctor of Philosophy, Case Western Reserve University, Biology.
In order to study the molecular genetics of factors controlling plant cell growth, a set of tumors on the small crucifer Arabidopsis thaliana was isolated and characterized. Seeds or seedlings were exposed to 60Co gamma radiation and, 30-60 days after germination, tumors developed either on the hypocotyl or in the region of the apical meristem of about 1% of the plants. When excised and placed in culture, some of these tumors were found to be capable of hormone-autonomous growth. The tumors exhibit a number of different phenotypes, varying in color, texture, and degree of differentiation. Some tumors appear to be completely undifferentiated, one consistently produces roots, and others show the sporadic appearance of shoots or leaflets. Growth rates of the tumors on hormone-free medium vary but are within the range of hormone-dependent callus and tumors induced by Agrobacterium. Investigations of gene expression in the tumors led to the identification of an mRNA that is abundant in all of the tumors and differentially expressed in plant organs and hormone-dependent callus grown on different auxins. The predicted translation product of the mRNA is a glycine-rich protein (GRP), and partial DNA sequence homology was found to previously characte rized GRP genes. Like other GRP sequences, this mRNA was found to be expressed in a tissue-specific manner, and accumulates in response to wounding or flooding stress. In an attempt to isolate the genomic sequences encoding the GRP mRNA, genomic clones were isolated using the GRP cDNA sequence as a probe. Comparison of one of the cloned genomic sequences with the cDNA revealed that the two sequences are not homologous but share short regions of GC-rich homology which are probably responsible for hybridization at normal stringency. Although the GRP sequence is not likely to play a role tumorigenesis, the use of the tumors in its identification illustrates how differences between the tumors and normal tissue can be exploited to study the regulation of genes of interest.
Christopher Town (Advisor)
136 p.
hormone autonomous tumors Arabidopsis thaliana

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Citations

  • Persinger, S. M. (1991). Isolation and characterization of hormone-autonomous tumors of Arabidopsis thaliana [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1055865093

    APA Style (7th edition)

  • Persinger, Sharon. Isolation and characterization of hormone-autonomous tumors of Arabidopsis thaliana. 1991. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1055865093.

    MLA Style (8th edition)

  • Persinger, Sharon. "Isolation and characterization of hormone-autonomous tumors of Arabidopsis thaliana." Doctoral dissertation, Case Western Reserve University, 1991. http://rave.ohiolink.edu/etdc/view?acc_num=case1055865093

    Chicago Manual of Style (17th edition)

case1055865093
598
© 1991, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.