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The role of DNA polymerase III in DNA repair and mutagenesis in Escherichia coli and Salmonella typhimurium

Slater, Steven Charles
http://rave.ohiolink.edu/etdc/view?acc_num=case1057261938

Abstract Details

1994, Doctor of Philosophy, Case Western Reserve University, Molecular Biology and Microbiology.
DNA polymerase III, the primary replicative polymerase of E. coli and S. typhimurium, is a large enzyme with multiple subunits. Its complexity reflects the complexity of its task: to accurately and rapidly replicate the approximately 4,700 Kb of DNA comprising the bacterial chromosome. Pol III interacts with numerous auxiliary proteins during initiation, elongation and termination, and with various SOS-induced proteins during error-prone repair. In none of these cases is the role of pol III completely delineated. The objective of this work was to genetically test the requirement for several pol III subunits, both during normal DNA replication and during SOS-mediated template lesion bypass. This has been accomplished using S. typhimurium dnaQ-null mutants that were previously constructed in this laboratory, and using E. coli dnaQ, holE, and holC, mutants constructed specifically for this study. The E. coli mutants were constructed using an allele exchange method I developed during this work. In addition, I constructed mucA and mucB alleles that were not dependent on recA for expression or activation. The work presented herein confirms the growth, mutagenesis, and suppression phenotypes associated with dnaQ-null mutations. In addition, it demonstrates that di sruption of holE has no obvious effect on cell viability, and that removal of holE and/or dnaQ does not impair the cell's ability to perform template lesion bypass. Finally, it shows that disruption of holC, which encodes the γ-complex χ subunit, has marked negative effects on DNA replication and cell viability, but that those effects are largely absent in strains bearing a suppressor mutation.
Russell Maurer (Advisor)
186 p.
DNA polymerase III DNA repair mutagenesis Escherichia coli Salmonella typhimurium

Recommended Citations

Citations

  • Slater, S. C. (1994). The role of DNA polymerase III in DNA repair and mutagenesis in Escherichia coli and Salmonella typhimurium [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1057261938

    APA Style (7th edition)

  • Slater, Steven. The role of DNA polymerase III in DNA repair and mutagenesis in Escherichia coli and Salmonella typhimurium. 1994. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1057261938.

    MLA Style (8th edition)

  • Slater, Steven. "The role of DNA polymerase III in DNA repair and mutagenesis in Escherichia coli and Salmonella typhimurium." Doctoral dissertation, Case Western Reserve University, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=case1057261938

    Chicago Manual of Style (17th edition)

case1057261938
485
© 1994, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.
Release 3.2.12