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Immune response to orthopaedic biomaterials

Yang, Jun.
http://rave.ohiolink.edu/etdc/view?acc_num=case1058195837

Abstract Details

1995, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
The use of metallic implants is playing an increasing role in the practice of orthopaedics. The implantation of each metallic device is associated with the release of metal either by corrosion and/or wear. As a result, patients might develop sensitivity to metallic constituents of the implant. To further our knowledge about the systemic immune system response to corrosion products of metallic orthopaedic devices, a series of experiments was done. 1) The saturation and competitive binding of chromium, cobalt, and nickel to serum protein (to form antigen) was determined. Nickel has highest affinity for protein. (2) A sensitive Enzyme Linked Immunosorbent Assay method for testing for antibody was developed. The serum samples of 10 patients with satisfactory F-75 total joint replacements (TJRs) were screened from samples obtained pre- and post-operatively for the presence of chromium, cobalt or nickel specific antibody. This experiment presents evidence that patients developed humoral immune responses after implantation of TJRs and metal-protein complex specific antibodies can be detected. (3) Serum samples from 10 patients with satisfactory TJRs and 15 patients with loose TJRs were tested. A high level of antibodies was detected in patients with loose TJRs. The antibody level of Ig G and Ig E against chromium were shown to be highly associated with the clinical TJR failure. (4) Leukocytes and serum samples obtained from 26 patients with loose total joint replacements were tested for the presence of the metal specific cell-mediated and humoral immunities. The finding suggested that both Ig G and Ig M antibodies against chromium-albumin complex increased significantly in the chromium cell mediated sensitive patients, while Ig E antibody against chromium decreased significantly. (5) To study the haptenic potential of these metal ions, a monoclonal antibody study was undertaken. Rabbit albumin-glutathione-metal complexes were injected into mice. Three mice which developed strong antibody reactions against GSH-metal were chosen for the production of monoclonal antibodies. Chromium, cobalt and nickel antibodies had similar affinity when they bound with the specific GSH-metal complex. There was very little cross reactivity between these antibodies. Thus high titer specific antibodies were produced. They were used to identify chromium, cobalt and nickel in the fretting corrosion products.
Katharine Merritt (Advisor)
257 p.
Immune response orthopaedic biomaterials

Recommended Citations

Citations

  • Yang, J. (1995). Immune response to orthopaedic biomaterials [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1058195837

    APA Style (7th edition)

  • Yang, Jun. Immune response to orthopaedic biomaterials. 1995. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1058195837.

    MLA Style (8th edition)

  • Yang, Jun. "Immune response to orthopaedic biomaterials." Doctoral dissertation, Case Western Reserve University, 1995. http://rave.ohiolink.edu/etdc/view?acc_num=case1058195837

    Chicago Manual of Style (17th edition)

case1058195837
621
© 1995, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.