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Investigation of Laminopathy-Like Alterations of the Nuclear Envelope caused by Accumulation of Esc1p

Hattier, Thomas
http://rave.ohiolink.edu/etdc/view?acc_num=case1138383986

Abstract Details

2006, Doctor of Philosophy, Case Western Reserve University, Pathology.
The possible existence of a lamina which determines nuclear shape in yeast is controversial since clear lamin sequence homologs do not exist. Nevertheless S. cerevisiae Esc1p resembles the lamins in that it contains coiled-coil domains and localizes to the nuclear periphery where it anchors heterochromatin. Increased expression of Esc1p causes dramatic elaborations of the nuclear envelope (NE), which extend into the cytoplasm and are reminiscent of modifications of nuclear shape seen in human laminopathies. These double membrane extensions, here referred to as "ESCapades", include nuclear pores, but little or no nucleoplasm. They generally contact the nucleolus, originate at the nucleus-vacuole junction and wrap around the vacuole. Examination of cells in anaphase shows that escapades are not inherited by daughter nuclei. Contrary to expectation, exclusion of these structures from daughters is independent of septin function and the presence of vacuoles. Moreover, they are excluded even when nuclear division is restricted to the maternal cytoplasm. Thus, this behavior may reflect the existence of an intrinsically coherent “maternal domain” of the nuclear periphery. The exclusion of atypical structures provides a striking example of “phenotypic normalization” which may make it possible to sustain continued cell growth when parental nuclei have acquired potentially deleterious characteristics. Consistent with this, retention of escapades slows the pace of bud formation in mothers while the overall speed of growth is only moderately affected.
Alan Tartakoff (Advisor)
185 p.
Biology, Cell
Esc1p; nuclear envelope; nuclear lamina; laminopathies; mRNP export

Recommended Citations

Citations

  • Hattier, T. (2006). Investigation of Laminopathy-Like Alterations of the Nuclear Envelope caused by Accumulation of Esc1p [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1138383986

    APA Style (7th edition)

  • Hattier, Thomas. Investigation of Laminopathy-Like Alterations of the Nuclear Envelope caused by Accumulation of Esc1p. 2006. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1138383986.

    MLA Style (8th edition)

  • Hattier, Thomas. "Investigation of Laminopathy-Like Alterations of the Nuclear Envelope caused by Accumulation of Esc1p." Doctoral dissertation, Case Western Reserve University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=case1138383986

    Chicago Manual of Style (17th edition)

case1138383986
1,109
© 2006, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.