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AUTOMATED SUB-MICRON RESOLUTION SERIAL BLOCK FACE IMAGING OF CANCELLOUS BONE USING EPIFLUORESCENCE MICROSCOPY

Slyfield, Craig R., Jr
http://rave.ohiolink.edu/etdc/view?acc_num=case1228370205

Abstract Details

2008, Master of Sciences (Engineering), Case Western Reserve University, EMC - Mechanical Engineering.
Osteoporosis is a disease characterized by high fracture risk and low bone mass. Bone mineral density is modified by a process known as bone remodeling. Bone remodeling is the resorption of bone by osteoclasts followed by deposition of new bone by osteoblasts. Because the resorption phase precedes formation, remodeling results in the formation of temporary cavities known as resorption cavities. Resorption cavities act as stress risers and are believed to impair fatigue properties and increase fracture risk. To better understand how bone remodeling affects the biomechanical properties of cancellous bone, images of bone and fluorescent markers of bone formation are collected through serial block face imaging. Images are collected using a computer numerically controlled mill with an attached fluorescence microscope. The techniques described here address factors contributing to imaging imprecision inherent to serial block face imaging using epifluorescence microscopy and allow direct measurement of resorption cavities.
Christopher Hernandez, PhD (Committee Chair)
Clare Rimnac, PhD (Committee Member)
Melissa Knothe-Tate, PhD (Committee Member)
87 p.
Biomedical Research; Computer Science; Electrical Engineering; Engineering; Mechanical Engineering
cancellous bone; bone; remodeling; imaging; serial; high resolution; fluorescence microscopy; markers; biomechanics; osteoporosis; medical imaging; engineering

Recommended Citations

Citations

  • Slyfield, Jr, C. R. (2008). AUTOMATED SUB-MICRON RESOLUTION SERIAL BLOCK FACE IMAGING OF CANCELLOUS BONE USING EPIFLUORESCENCE MICROSCOPY [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1228370205

    APA Style (7th edition)

  • Slyfield, Jr, Craig. AUTOMATED SUB-MICRON RESOLUTION SERIAL BLOCK FACE IMAGING OF CANCELLOUS BONE USING EPIFLUORESCENCE MICROSCOPY. 2008. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1228370205.

    MLA Style (8th edition)

  • Slyfield, Jr, Craig. "AUTOMATED SUB-MICRON RESOLUTION SERIAL BLOCK FACE IMAGING OF CANCELLOUS BONE USING EPIFLUORESCENCE MICROSCOPY." Master's thesis, Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1228370205

    Chicago Manual of Style (17th edition)

case1228370205
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© 2008, some rights reserved.Creative Commons License AUTOMATED SUB-MICRON RESOLUTION SERIAL BLOCK FACE IMAGING OF CANCELLOUS BONE USING EPIFLUORESCENCE MICROSCOPY by Craig R. Slyfield Jr is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.