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Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II

Zhao, Fan
http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860

Abstract Details

2010, Master of Sciences, Case Western Reserve University, Biochemistry.
Glycosylation of immune proteins plays critical roles in the adaptive immune system. Complex asparagine (N)-linked glycans are involved in directing the proper protein folding and trafficking to the cell surface, protection from the proteolytic cleavage, and promoting the appropriate cell-cell adhesion and communication. Our study shows that elimination of complex N-linked glycans on MHC II reduced the presentation of a novel zwitterionic polysaccharide antigen PSA which expressed by B. fragilis but not conventional peptides in live antigen presenting cells (APCs). The binding assay demonstrated that full size complex N-glycans are necessary for PSA but not conventional peptide antigen binding to MHC II. T cell activation assay conducted in mouse bone marrow-derived dendritic cells (BMDCs) shows the in vitro evidence that PSA-mediated T cell activation is significantly reduced due to the blockade of complex N-glycans of MHC II in APCs. However, lacking N-glycans on MHC II does not affect OT-II T cell activation with OVA peptide. These results reveal that N-glycosylation of MHC II directly influences PSA but not conventional protein antigen presentation and downstream T cell activation.
William A. Merrick, PhD (Committee Chair)
Brian A. Cobb, PhD (Advisor)
Thomas Gerken, PhD (Committee Member)
65 p.
Immunology
MHC II; Glycosylation; Carbohydrate Antigen Presentation

Recommended Citations

Citations

  • Zhao, F. (2010). Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860

    APA Style (7th edition)

  • Zhao, Fan. Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II. 2010. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860.

    MLA Style (8th edition)

  • Zhao, Fan. "Complex N-glycans are Required for Carbohydrate Antigen Presentation by MHC II." Master's thesis, Case Western Reserve University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1278621860

    Chicago Manual of Style (17th edition)

case1278621860
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© 2010, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.