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Magnesium Regulation of Glucose and Fatty Acid Metabolism in HEPG2 Cells

Etwebi, Zienab
http://rave.ohiolink.edu/etdc/view?acc_num=case1307564164

Abstract Details

2011, Master of Sciences, Case Western Reserve University, Physiology and Biophysics.
Magnesium (Mg2+) is an important cation for a variety of cell functions such as enzyme activity, nucleic acid and protein synthesis, and energy metabolism. Mg2+ deficiency has been correlated with the onset and progression of several pathological conditions including diabetes, insulin resistance, metabolic syndrome and obesity. Several studies looking at Mg2+ homeostasis show that Mg2+ homeostasis is associated with glucose metabolism and is inversely correlated with lipid metabolism. Exposure to Mg2+ deficient diet results in a noticeable decrease in hepatic glucose accumulation and a two- fold increase in intrahepatic triglyceride content. In this study, we investigated the effect of changes in Mg2+ concentrations inside and/or outside the liver cell on glucose uptake in the absence and in the presence of insulin stimulation. Our results show that low extracellular Mg2+ content impairs insulin stimulated glucose uptake, with no significant effects on glucose transporters expression. In the case of lipid metabolism, low extracellular Mg2+ content or Mg2+ deficiency decreases the levels of SREBP-1c precursor, an insulin dependent transcription factor, further corroborating the involvement of Mg2+ in modulating hepatic response to insulin. Mg2+ deficiency also decreases the expression level of PPARα, a transcriptional factor involved in fatty acid oxidation. Lastly, Mg2+ deficiency upregulates SREBP2, and PPARγ, transcription factors that are both involved in increasing fatty acid synthesis in liver cells. All together our data indicate that extracellular and cellular Mg2+ levels are important in modulating glucose uptake and fatty acids metabolism in liver cells.
ANDREA ROMANI (Advisor)
89 p.
Physiology

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Citations

  • Etwebi, Z. (2011). Magnesium Regulation of Glucose and Fatty Acid Metabolism in HEPG2 Cells [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1307564164

    APA Style (7th edition)

  • Etwebi, Zienab. Magnesium Regulation of Glucose and Fatty Acid Metabolism in HEPG2 Cells. 2011. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1307564164.

    MLA Style (8th edition)

  • Etwebi, Zienab. "Magnesium Regulation of Glucose and Fatty Acid Metabolism in HEPG2 Cells." Master's thesis, Case Western Reserve University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1307564164

    Chicago Manual of Style (17th edition)

case1307564164
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© 2011, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.