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PISP: A Novel Component of the Apical Barrier Formed Between Hair Cells and Supporting Cells in the Inner Ear Sensory Epithelia

Gupta, Harshita
http://rave.ohiolink.edu/etdc/view?acc_num=case1329232107

Abstract Details

2012, Master of Sciences, Case Western Reserve University, Biology.
Disturbances to ionic barrier formed at apical regions of the sensory epithelia, between hair cells and supporting cells, cause changes in electrical properties of the inner ear, resulting in severe hearing loss and vestibular dysfunction. PDZ domain proteins mediate barrier formation through their occluding and adhesive properties. PISP, a single PDZ- domain protein, was identified in a yeast two-hybrid screen using human brain cDNA library and in a microarray analysis of the zebrafish hair cell transcriptome. We here confirm expression of pisp mRNA in zebrafish and show PISP protein expression in mouse inner ear. Interaction of zebrafish Pisp and Pmca2b orthologs was observed in vitro. In vivo, PISP localized to areas of cell-cell contact between hair cells and supporting cells in chicken and mouse sensory epithelia. Our results thus identify PISP as a novel component of the barrier formed between hair cells and supporting cells in the inner ear sensory epithelia.
Brian M. McDermott, PhD (Advisor)
Robin Snyder, PhD (Committee Chair)
Emmitt R. Jolly, PhD (Committee Member)
Heather Broihier, PhD (Committee Member)
Biology
hair cell; junction; PISP; reticular lamina; sensory epithelium; hearing;

Recommended Citations

Citations

  • Gupta, H. (2012). PISP: A Novel Component of the Apical Barrier Formed Between Hair Cells and Supporting Cells in the Inner Ear Sensory Epithelia [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1329232107

    APA Style (7th edition)

  • Gupta, Harshita. PISP: A Novel Component of the Apical Barrier Formed Between Hair Cells and Supporting Cells in the Inner Ear Sensory Epithelia. 2012. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1329232107.

    MLA Style (8th edition)

  • Gupta, Harshita. "PISP: A Novel Component of the Apical Barrier Formed Between Hair Cells and Supporting Cells in the Inner Ear Sensory Epithelia." Master's thesis, Case Western Reserve University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=case1329232107

    Chicago Manual of Style (17th edition)

case1329232107
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© 2012, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.