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073012_Final Thesis_Irene Tsung.pdf (4.19 MB)

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INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS

Tsung, Irene
http://rave.ohiolink.edu/etdc/view?acc_num=case1363689452

Abstract Details

2013, Master of Sciences, Case Western Reserve University, Biomedical Engineering.
Therapeutic vascularization remains a challenge in the field of tissue engineering. Designing tissues for long-term success involves finding methods to incorporate functional vascular networks capable of providing nutrients and oxygen and removing wastes from surrounding cells. Recent advances have explored synthetic hydrogel scaffolds aimed at mimicking the ECM to create prevascularized tissues. These hydrogels include cell adhesion peptides, enzyme degradable sequences, and growth factors for endothelial cell homing, matrix remodeling, and proliferation. In order to tune scaffold properties for optimal angiogenic-promoting conditions, material parameters must be adjusted. In this project, a cellular platform was generated to probe a single aspect of the system, enzyme degradable sequences. Cells capable of controllable, inducible expression of Mmp1a were cloned for optimization of collagenase degradable sequence function in PEG-based hydrogels. These cells overexpressed Mmp1a mRNA by QPCR analysis, though further experiments in Western blot and zymography need refining for detecting protein presence and activity.
Horst von Recum (Committee Chair)
Paul Tesar (Committee Member)
Roger Marchant (Committee Member)
172 p.
Biomedical Engineering; Engineering

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Citations

  • Tsung, I. (2013). INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1363689452

    APA Style (7th edition)

  • Tsung, Irene. INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS. 2013. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1363689452.

    MLA Style (8th edition)

  • Tsung, Irene. "INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS." Master's thesis, Case Western Reserve University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=case1363689452

    Chicago Manual of Style (17th edition)

case1363689452
769
© 2012, some rights reserved.Creative Commons License INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS by Irene Tsung is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.