Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Thesis Format check- edits 2.pdf (1.19 MB)

ETD Abstract Container

Abstract Header

Investigating the Low Adiposity of Cystic Fibrosis Mice

Klavanian, Jeannie
http://rave.ohiolink.edu/etdc/view?acc_num=case1401902536

Abstract Details

2014, Master of Sciences, Case Western Reserve University, Genetic Counseling.
Background: Cystic fibrosis (CF) patients and mice have low amounts of adipose tissue. The purpose of this study was to determine whether this adipose tissue deficiency is due to fewer adipocytes, smaller adipocytes, or both. Methods: Studies were performed on F508del (Cftrtm1kth) mice and wild-type (WT) littermates. Gonadal fat pads were excised and weighed. DNA and triglycerides were extracted from fat pads and quantified using PicoGreen and colorimetric assays. Cell number and density were estimated from histological sections of CF and WT adipose tissue. Results: CF mouse adipocytes in inguinal fat pads were found to be similar in number between CF and non-CF samples, as both had comparable DNA content, but histological assessment showed that CF cells were clearly smaller than non-CF adipocytes. CF adipocytes had less triglyceride when normalized to DNA content. Conclusions: CF mice have smaller adipocytes but similar total number of adipocytes compared to WT mice.
Rebecca Darrah (Committee Chair)
Ilya Bederman (Committee Member)
Mitchell Drumm (Committee Member)
Anne Matthews (Committee Member)
63 p.
Biology; Genetics
Cystic fibrosis; adipose tissue; adipocyte size; adipose tissue histology

Recommended Citations

Citations

  • Klavanian, J. (2014). Investigating the Low Adiposity of Cystic Fibrosis Mice [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1401902536

    APA Style (7th edition)

  • Klavanian, Jeannie. Investigating the Low Adiposity of Cystic Fibrosis Mice. 2014. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1401902536.

    MLA Style (8th edition)

  • Klavanian, Jeannie. "Investigating the Low Adiposity of Cystic Fibrosis Mice." Master's thesis, Case Western Reserve University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1401902536

    Chicago Manual of Style (17th edition)

case1401902536
343
© 2014, some rights reserved.Creative Commons License Investigating the Low Adiposity of Cystic Fibrosis Mice by Jeannie Klavanian is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.