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Kelley Carr Master Thesis Final.pdf (3.45 MB)

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The Mechanism of the Prolonged Action of the Single-Chain Insulin, 70-01

Carr, Kelley
http://rave.ohiolink.edu/etdc/view?acc_num=case1511982660839103

Abstract Details

, Master of Sciences, Case Western Reserve University, Biochemical Research.
Insulin plays a key role in metabolism. Insulin binds to its insulin receptors on insulin responsive cell surfaces and stimulates cellular uptake of glucose, turns on glycolysis and glycogen synthesis, inhibits gluconeogenesis and glycogenolysis in the liver, and stimulates triglyceride synthesis. Previous research has shown that amino acid substitutions, deletions, or additions on insulin alter insulin function. We have found the single-chain insulin (SCI) 70-01, with a substitution of B10His to Asp (12,16), causes blood glucose to fall as rapidly as KP but remain lower longer when injected in diabetic rats. The focus of this research is to study the mechanism of 70-01’s prolonged action, and compare it to control insulins, including 81-01, an identical SCI devoid of the AspB10 substitution. We conclude that 70-01 remains for a longer period in the bloodstream, and continues to stimulate phosphorylation of the insulin receptors in a tissue-specific manner, longer than control insulins.
Faramarz Ismail-Beigi (Advisor)
William Merrick (Committee Chair)
Martin Snider (Committee Member)
Biochemistry
diabetes; insulin; insulin receptor; AKT; insulin signaling

Recommended Citations

Citations

  • Carr, K. (n.d.). The Mechanism of the Prolonged Action of the Single-Chain Insulin, 70-01 [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1511982660839103

    APA Style (7th edition)

  • Carr, Kelley. The Mechanism of the Prolonged Action of the Single-Chain Insulin, 70-01. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1511982660839103.

    MLA Style (8th edition)

  • Carr, Kelley. "The Mechanism of the Prolonged Action of the Single-Chain Insulin, 70-01." Master's thesis, Case Western Reserve University. Accessed MAY 21, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=case1511982660839103

    Chicago Manual of Style (17th edition)

case1511982660839103
231
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This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.