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Wanli Thesis (1).pdf (2.48 MB)

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Development of an injectable gelatin material formulation for cartilage tissue engineering

Ma, Wanli
http://rave.ohiolink.edu/etdc/view?acc_num=case1516621877430542

Abstract Details

2018, Master of Sciences (Engineering), Case Western Reserve University, EMC - Mechanical Engineering.
A large number of musculoskeletal conditions, including arthritis lead to the demand for the development of tissue engineering. The suitable biomaterial is the key to the success of the tissue engineering. In this work, an injectable gelatin material for cartilage tissue engineering was developed. Since the mechanical properties and degradation of uncross-linked gelatin cannot fulfill the requirements of tissue engineering, five crosslinking methods (UV, Rose Bengal, EDC/NHS, Genipin, Transglutaminase-TG) were screened in terms of their efficacy in improving the mechanical and degradation properties of gelatin. After crosslinking, mechanical test, degradation test, and cell viability tests were conducted. The effect of air spraying of cell-gel mixtures on cell viability was also investigated. Transglutaminase was found to maintain cell-viability while providing stiffness and extending degradation of gelation. Such that, the modulus of TG cross-linked gelatin hydrogel 74 ± 6 kPa. On the other hand, the degradation time was 12 days, which is still not sufficient for long-time cell encapsulation. Therefore, while TG crosslinking provides baseline promise in terms of initial modulus and cell viability, additional formulation changes will be required to extend the degradation timeline.
Ozan Akkus (Committee Chair)
Umut Gurkan (Committee Member)
Clare Rimnac (Committee Member)
86 p.
Biomechanics; Mechanical Engineering
tissue engineering; gelatin; crosslinking; transglutaminase

Recommended Citations

Citations

  • Ma, W. (2018). Development of an injectable gelatin material formulation for cartilage tissue engineering [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1516621877430542

    APA Style (7th edition)

  • Ma, Wanli. Development of an injectable gelatin material formulation for cartilage tissue engineering. 2018. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1516621877430542.

    MLA Style (8th edition)

  • Ma, Wanli. "Development of an injectable gelatin material formulation for cartilage tissue engineering." Master's thesis, Case Western Reserve University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1516621877430542

    Chicago Manual of Style (17th edition)

case1516621877430542
736
© 2018, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.