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Thesis for Hao Wang.pdf (2.92 MB)

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THE POTENTIAL INDUCING PATTERN OF THE FLAX GENOME

Wang, Hao
http://rave.ohiolink.edu/etdc/view?acc_num=case1532609009820723

Abstract Details

2019, Master of Sciences, Case Western Reserve University, Biology.
Previous studies have shown that certain flax varieties are especially prone to genomic destabilization by the external growing environment. The best characterized variety that responds to this environmental stress is the fiber flax variety Stormont cirus, which is subsequently referred to Pl. Bethune is an oil seed variety, which appears to be genomically stable irrespective of the growth environment. To elucidate the genetic control of this phenomenon, a cross was made between Pl and Bethune. The F1 generation from this cross (Bethune x Pl) was grown in a non-inducing environment and appeared to be stable under this environment. However, when the F1 generation was grown under an inducing environment, then genomic variation was observed which is consistent with the character of inducibility under the stress of the external growing environment being a dominant characteristic. When F2 individuals from these non-induced F1’s were grown under inducing conditions then, using the amplification of LIS-1 as the marker for inducibility, some individuals showed inducibility and some were stable. Scoring many loci in these induced F2’s showed that many were inherited in the expected Mendelian pattern, but some were inherited anomalously. In general, the data indicated that there was an overabundance of Bethune parental alleles, where the allele in Bethune was the same as that in induced progeny from Pl. The alleles were characterized by PCR so individual loci were scored independently. Subsequently, whole genome sequencing was done for a set of DNAs isolated from these F2’s. A 2Mb region of Chromosome 14 was selected because it is a potential region which contains multiple sections that respond to the growth environment. The rDNA, which is a quantitative polymorphism associated with the induction of genomic changes, was also assessed to observe the data for a different chromosome. Several potential blocks and some SNPs have been determined as the border of responsive regions. The rDNA data shows a different extent of responsiveness in F2 individuals and indicates that the induction may be under the control of 2 genes.
Christopher Cullis (Advisor)
Michael Benard (Committee Chair)
Sarah Bagby (Committee Member)
Peter Zimmerman (Committee Member)
64 p.
Bioinformatics; Biology
PCR, LIS-1, Ribosomal RNA genes, Genome destabilization, Sequence comparison, bioinformatics analyses

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Citations

  • Wang, H. (2019). THE POTENTIAL INDUCING PATTERN OF THE FLAX GENOME [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1532609009820723

    APA Style (7th edition)

  • Wang, Hao. THE POTENTIAL INDUCING PATTERN OF THE FLAX GENOME. 2019. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1532609009820723.

    MLA Style (8th edition)

  • Wang, Hao. "THE POTENTIAL INDUCING PATTERN OF THE FLAX GENOME." Master's thesis, Case Western Reserve University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1532609009820723

    Chicago Manual of Style (17th edition)

case1532609009820723
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This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.