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An Approach to Lens Regeneration in Mice Following Lentectomy and the Implantation of a Biodegradable Hydrogel Encapsulating Iris Pigmented Tissue in Combination with Basic Fibroblast Growth Factor

Baddour, Joelle
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1335916825

Abstract Details

2012, Master of Science (M.S.), University of Dayton, Chemical Engineering.
Organ or tissue regeneration is the process by which damaged or lost tissue parts or whole body organs are repaired or replaced. When compared to amphibians, mammals possess very limited regenerative capabilities. Mammals are capable of lens regeneration following lentectomy only if the lens capsule is left behind. Regeneration is achieved by the residual lens epithelial cells (LECs) adherent to the remaining lens capsule. Urodele amphibians, however, have been reported to regenerate their lenses, following whole organ removal, by the transdifferentiation of the pigmented epithelial cells (PECs) of the dorsal iris. These cells, namely PECs, have been shown to possess a potential for transdifferentiation in vitro as well as in vivo. In this study, the feasibility of coaxing iris PECs to regenerate a lens in vivo was tested by encapsulating an iris pigmented epithelial tissue by a hydrogel bead combined with FGF and implanting the resulting matrix in lentectomized mice. This study also investigates the ability of aligned Poly-ε-caprolactone (PCL) nanofibers in inducing the differentiation of LECs and the subsequent alignment of lens fiber cells.
Panagiotis A Tsonis, PhD (Committee Chair)
Robert J Wilkens, PhD (Committee Member)
Amit Singh, PhD (Committee Member)
171 p.
Biology; Biomedical Engineering; Biomedical Research; Chemical Engineering; Developmental Biology
Lens Regeneration; Iris Pigmented Epithelial Cells; Lens Epithelial Cells; Transdifferentiation; Differentiation; Fibroblast Growth Factor (FGF); Hydrogel; Poly-&949; -caprolactone (PCL); Nanofibers; Mouse

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Citations

  • Baddour, J. (2012). An Approach to Lens Regeneration in Mice Following Lentectomy and the Implantation of a Biodegradable Hydrogel Encapsulating Iris Pigmented Tissue in Combination with Basic Fibroblast Growth Factor [Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1335916825

    APA Style (7th edition)

  • Baddour, Joelle. An Approach to Lens Regeneration in Mice Following Lentectomy and the Implantation of a Biodegradable Hydrogel Encapsulating Iris Pigmented Tissue in Combination with Basic Fibroblast Growth Factor. 2012. University of Dayton, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1335916825.

    MLA Style (8th edition)

  • Baddour, Joelle. "An Approach to Lens Regeneration in Mice Following Lentectomy and the Implantation of a Biodegradable Hydrogel Encapsulating Iris Pigmented Tissue in Combination with Basic Fibroblast Growth Factor." Master's thesis, University of Dayton, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1335916825

    Chicago Manual of Style (17th edition)

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© 2012, all rights reserved.
This open access ETD is published by University of Dayton and OhioLINK.