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Hawkins_Caitlin_Thesis_Manuscript.pdf (2.45 MB)

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Development of a FRET-based assay to determine binding affinities of RsmG to 30S 5'-domain RNA-protein complexes

Hawkins, Caitlin Marie
http://orcid.org/0000-0003-3306-4994
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1557447007620285

Abstract Details

2019, BS, Kent State University, College of Arts and Sciences / Department of Chemistry and Biochemistry.
Ribosomes are the molecular machines that carry out protein biosynthesis in all living organisms. They are composed of three different ribosomal RNAs and more than 50 ribosomal proteins. The thermodynamics and kinetics of in vitro ribosomal assembly have been studied extensively. However, during ribosome biogenesis, in vivo ribosome assembly occurs concurrently with transcription, folding, post-transcriptional modifications, and processing of rRNA. Unfortunately, the effects post-transcriptional RNA modification enzymes on ribosomal assembly are understudied. My project in Abey lab is to investigate how RNA modification enzyme RsmG influences 30S bacterial ribosome assembly. I have developed a FRET based assay to monitor binding of RsmG to ribosomal RNA, which will allow us to determine the binding affinity of RsmG to RNA and thus calculate thermodynamic cooperativity between RsmG enzyme and ribosomal proteins. Our findings give us more insight to how modification enzymes modulate the hierarchy of protein addition during ribosome biogenesis.
Sanjaya Abeysirigunawardena, PhD (Advisor)
Gail Fraizer, PhD, MPH (Committee Member)
Hanbin Mao, PhD (Committee Member)
Alexander Seed, PhD (Committee Member)
51 p.
Biochemistry
RsmG, bacterial ribosome assembly, FRET, binding affinity, modification enzyme

Recommended Citations

Citations

  • Hawkins, C. M. (2019). Development of a FRET-based assay to determine binding affinities of RsmG to 30S 5'-domain RNA-protein complexes [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1557447007620285

    APA Style (7th edition)

  • Hawkins, Caitlin. Development of a FRET-based assay to determine binding affinities of RsmG to 30S 5'-domain RNA-protein complexes. 2019. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1557447007620285.

    MLA Style (8th edition)

  • Hawkins, Caitlin. "Development of a FRET-based assay to determine binding affinities of RsmG to 30S 5'-domain RNA-protein complexes." Undergraduate thesis, Kent State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1557447007620285

    Chicago Manual of Style (17th edition)

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© 2019, all rights reserved.
This open access ETD is published by Kent State University Honors College and OhioLINK.