Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List

Full text of this paper is not available in the ETD Center. Copies may be available for inter-library loan from Kent State University or may be available for purchase from Proquest/UMI

ETD Abstract Container

Abstract Header

Characterization of a novel peptide inhibitor of RsmC function

To, Davidnhan D
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1558543430558686

Abstract Details

2019, BS, Kent State University, College of Arts and Sciences / Department of Chemistry and Biochemistry.
Within the past century, several strains of bacteria have developed resistance to common antibiotics, such as methicillin and vancomycin, making it increasingly difficult to treat and prevent infections. Thus, development of novel antibiotics has become a necessity. The goal of our research is to disrupt ribosomal assembly mechanisms by inhibiting ribosomal modification enzymes. Protein RsmC (ribosomal RNA small subunit methyltransferase C), which binds to the 3’ helix 34 (3’-h34) of the 16S 3’-major domain rRNA and methylates a guanine at position 1207 (G1207; E. coli numbering), is the focus of this research. Transverse mutations at position 1207 resulted in a lethal phenotype, perhaps due to the formation of a nonfunctioning ribosome. We have discovered a 7-mer peptide that binds tightly to 3’-h34 RNA using phage display. Tryptophan quenching experiments confirmed the binding of the peptide to 3’-h34, in addition to several 3’-h34 model RNAs. Furthermore, the binding of the peptide to the RNA was found to be sequence dependent. Circular dichroism was used to verify the structure of the model RNAs during the quenching experiments.
Sanjaya Abeysirigunawardena (Advisor)
Soumitra Basu (Committee Member)
Gary Koski (Committee Member)
Suzy D'Enbeau (Committee Member)
48 p.
Biochemistry
antibiotic resistance, tryptophan, circular dichroism

Recommended Citations

Citations

  • To, D. D. (2019). Characterization of a novel peptide inhibitor of RsmC function [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1558543430558686

    APA Style (7th edition)

  • To, Davidnhan. Characterization of a novel peptide inhibitor of RsmC function. 2019. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1558543430558686.

    MLA Style (8th edition)

  • To, Davidnhan. "Characterization of a novel peptide inhibitor of RsmC function." Undergraduate thesis, Kent State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1558543430558686

    Chicago Manual of Style (17th edition)

ksuhonors1558543430558686