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Effectiveness of Fluorogold Bound Conjugate in Imaging Mice Neuroendocrine Circuits

Abstract Details

2020, BS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
The purpose of this study was to analyze the efficacy of a fluorogold bound conjugate in labeling neuroendocrine circuits of the median eminence and paraventricular nucleus in the hypothalamus. Fluorogold is a retrograde tracer that is detectable by confocal microscopy and can be linked to gadolinium DOTA to be detectable by MRI. The fluorogold bound conjugate labels neuroendocrine cells with axon terminals that extend beyond the blood-brain barrier following an intraperitoneal injection. 72 hours after injection, MR images were taken then mice were sacrificed and brains were extracted and sliced to be analyzed with confocal microscopy. Results show that the fluorogold bound nanoparticle was not as effective as fluorogold alone in labeling the median eminence or paraventricular nucleus hypothalamus, but there was off-target labeling in TRH neurons of the dorsal parvocellular paraventricular nucleus. This study establishes a framework for future creation of more effective particles that can be detectable by both confocal microscopy and MRI. This data will be used to understand mechanisms and to identify potential techniques to image neuroendocrine cells noninvasively, especially in the hypothalamus, as they are important for hormonal regulation of important functions within the body and dysfunction can lead to issues such as neurodegenerative disease, cardiac and respiratory dysfunction, and blood pressure abnormalities.
Robert Clements (Advisor)
Jennifer McDonough (Committee Member)
Elda Hegmann (Committee Member)
29 p.

Recommended Citations

Citations

  • Riley, A. L. (2020). Effectiveness of Fluorogold Bound Conjugate in Imaging Mice Neuroendocrine Circuits [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1587996298161636

    APA Style (7th edition)

  • Riley, Amanda. Effectiveness of Fluorogold Bound Conjugate in Imaging Mice Neuroendocrine Circuits. 2020. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1587996298161636.

    MLA Style (8th edition)

  • Riley, Amanda. "Effectiveness of Fluorogold Bound Conjugate in Imaging Mice Neuroendocrine Circuits." Undergraduate thesis, Kent State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1587996298161636

    Chicago Manual of Style (17th edition)