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Effects of Mononitroparaben on Lipid Content of Melanoma Cells

Schlanz, Julie Ann
http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1683297844540949

Abstract Details

2023, Bachelor of Science, Marietta College, Chemistry.
Parabens are commonly used as preservatives in regularly used topical products, but their safety is under discussion since small amounts of paraben have been found in tumor tissue. Mononitroparaben causes cell death in melanoma cells with an LC50 value of 7.02mM after twelve hours of treatment. This study focused on how mononitroparaben affects the cellular lipid content during induced cell death. The experiment was conducted by growing the M624 melanoma cells, dissolving the paraben in methanol, and then leaving 0 mM, 5 mM, and 10 mM concentrations of paraben on the melanoma cancer cells for twelve hours. The paraben was then removed from the cells, and the cells were lysed. Colorimetric cholesterol assays and ceramide assays were completed to determine the changes in cellular cholesterol and ceramide content and the role of cellular lipids in cell death signaling. The results showed that cholesterol concentrations did not significantly change among cells treated with mononitroparaben. However, ceramide significantly increased for cells treated with 10 mM mononitroparaben, which indicated that apoptosis occurred.
Suzanne Parsons, Ph.D. (Advisor)
Adam Jacoby, Ph.D. (Committee Member)
Rakibul Sarker, Ph.D. (Committee Member)
48 p.
Biochemistry
parabens; paraben; mononitroparaben; lipids; melanoma cells; cholesterol; ceramide; cell death; apoptosis

Recommended Citations

Citations

  • Schlanz, J. A. (2023). Effects of Mononitroparaben on Lipid Content of Melanoma Cells [Undergraduate thesis, Marietta College]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1683297844540949

    APA Style (7th edition)

  • Schlanz, Julie. Effects of Mononitroparaben on Lipid Content of Melanoma Cells. 2023. Marietta College, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1683297844540949.

    MLA Style (8th edition)

  • Schlanz, Julie. "Effects of Mononitroparaben on Lipid Content of Melanoma Cells." Undergraduate thesis, Marietta College, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1683297844540949

    Chicago Manual of Style (17th edition)

marhonors1683297844540949
46
© 2023, all rights reserved.
This open access ETD is published by Marietta College Honors Theses and OhioLINK.