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Identification of Francisella tularensis Outer Membrane Proteins

Melillo, Amanda Adeline
http://rave.ohiolink.edu/etdc/view?acc_num=mco1121867713

Abstract Details

2005, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Graduate Studies.
Francisella tularensis is a Gram-negative bacterium that causes tularemia. It is a potential biological weapon because of its extreme infectivity (<10 organisms), ease of dissemination, and substantial capacity to cause illness and death. Little is known about the pathogenesis of F. tularensis infections. We hypothesize that adherence of F. tularensis to lung epithelium is an important first step in pathogenesis and that surface proteins (adhesins) are critical for this adherence. We have observed enhanced adherence of F. tularensis LVS (live vaccine strain) to the human epithelial cell line, A549, when compared to the adherence of subsp. novicida or laboratory strains of E. coli. To identify surface proteins, bacteria were treated with a membrane impermeable biotinylation agent. We have identified one known F. tularensis surface protein (FopA) and seven putative outer membrane proteins. This work will give us a greater understanding of the initial steps in F. tularensis pathogenesis.
Eric Lafontaine, Ph.D. (Advisor)
96 p.
Biology, Microbiology
Francisella tularensis; Outer Membrane Proteins; Biodefense

Recommended Citations

Citations

  • Melillo, A. A. (2005). Identification of Francisella tularensis Outer Membrane Proteins [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1121867713

    APA Style (7th edition)

  • Melillo, Amanda. Identification of Francisella tularensis Outer Membrane Proteins. 2005. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1121867713.

    MLA Style (8th edition)

  • Melillo, Amanda. "Identification of Francisella tularensis Outer Membrane Proteins." Master's thesis, University of Toledo, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=mco1121867713

    Chicago Manual of Style (17th edition)

mco1121867713
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© 2005, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.