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The Effects of Cardiotonic Steroids on Dermal Collagen Synthesis and Wound Healing

El-Okdi, Nasser Samir
http://rave.ohiolink.edu/etdc/view?acc_num=mco1213732499

Abstract Details

2008, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Graduate Studies.
We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na/K-ATPase pathway. In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid, marinobufagenin, in a dose and time dependent-fashion. Next, we examined the effect of digoxin, ouabain and marinobufagenin on the rate of wound closure in an in vitro model where human dermal fibroblasts cultures were wounded with a pipette tip and monitored by digital microscopy. Finally, we administered digoxin in an in vivo wound healing model, using olive oil as a vector. This application significantly accelerated in vivo wound healing in rats wounded with an 8 mm biopsy cut. Increased collagen accumulation was noted 9 days after wounding (both p < 0.01). These data suggest that cardiotonic steroids induce increases in collagen synthesis by dermal fibroblasts, and accelerate wound healing.
Joseph Shapiro, MD (Committee Chair)
Sonia Najjar, PhD (Committee Member)
Zi-Jian Xie, PhD (Committee Member)
Deepak Malhotra, MD, PhD (Committee Member)
66 p.
Molecular Biology; Pharmacology; Surgery
collagen; fibroblast; cardiotonic steroids; wound healing; Na/K-ATPase

Recommended Citations

Citations

  • El-Okdi, N. S. (2008). The Effects of Cardiotonic Steroids on Dermal Collagen Synthesis and Wound Healing [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1213732499

    APA Style (7th edition)

  • El-Okdi, Nasser. The Effects of Cardiotonic Steroids on Dermal Collagen Synthesis and Wound Healing. 2008. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1213732499.

    MLA Style (8th edition)

  • El-Okdi, Nasser. "The Effects of Cardiotonic Steroids on Dermal Collagen Synthesis and Wound Healing." Master's thesis, University of Toledo, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1213732499

    Chicago Manual of Style (17th edition)

mco1213732499
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© 2008, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.