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Analysis of Rat Chromosome 9 for Genetic Determinants of Blood Pressure

Saikumar, Jagannath H.
http://rave.ohiolink.edu/etdc/view?acc_num=mco1213790607

Abstract Details

2008, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Graduate Studies.
BP QTL on the Rat Chromosome 9(RNO9) have been previously shown to have a significant BP lowering effect when trapped in congenic strains developed by selective backcrossing of the Dahl salt-sensitive (S) and Dahl salt-resistant (R) strain. One such S.R(9) congenic strain, S.R(9)x3x2C had a significantly lower BP than the S rat and this effect was lost in the S.R(9)x3x2Cx1 sub-strain derived from the S.R(9)x3x2C strain. The R alleles trapped in the S.R(9)x3x2Cx1 strain were also a part of the S.R(9)x3x2Bx1 strain which is an adjoining congenic strain also having a significantly lower BP compared to the S rat. These differences were attributed to the genetic elements trapped in the congenic strains. To test this hypothesis and to examine if there were any interactions between the genetic elements or the now fragmented parts of the BP QTL, real-time gene expression of the genes in the overlapping part of the three congenic strains were performed. Significant differences in the expression were found suggesting a complex mechanism for the BP control in this region. Differential atrial ANP content between S and R rats is clearly documented at a young age of 4-6 weeks. Since the relationship between the genetic factors dictating atrial ANP content and the genetic factors controlling BP (BP) is not known, these congenic strains were tested for atrial ANP content at two time points -4 weeks and 1 day neonatal animals. Significant differences were found between the different strains and matched the trend in the BP measured in the strains. This provides evidence to the link between atrial ANP content and BP in two possible ways, as a sensitive marker at a pre-hypertensive age for future hypertension and as a major phenomenon for genetically controlled pathogenesis for hypertension.
Bina Joe, PhD (Committee Chair)
Sumudra Periyasamy, PhD (Committee Member)
David Giovannucci, PhD (Committee Member)
85 p.
Molecular Biology
rat; congenic; chromosome; genetic; hypertension

Recommended Citations

Citations

  • Saikumar, J. H. (2008). Analysis of Rat Chromosome 9 for Genetic Determinants of Blood Pressure [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1213790607

    APA Style (7th edition)

  • Saikumar, Jagannath. Analysis of Rat Chromosome 9 for Genetic Determinants of Blood Pressure. 2008. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1213790607.

    MLA Style (8th edition)

  • Saikumar, Jagannath. "Analysis of Rat Chromosome 9 for Genetic Determinants of Blood Pressure." Master's thesis, University of Toledo, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1213790607

    Chicago Manual of Style (17th edition)

mco1213790607
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© 2008, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.