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Identification, Characterization, and Ontogenic Study of Three Novel Zebrafish Cytosolic Sulfotransferases (SULTs)

Mohammed, Yasir Ihsan
http://rave.ohiolink.edu/etdc/view?acc_num=mco1303486828

Abstract Details

2011, Master of Science in Pharmaceutical Science (MSP), University of Toledo, College of Pharmacy.
In mammals, sulfation as catalyzed by the cytosolic sulfotransfereases (SULTs) is known to be involved in the metabolism and homeostasis of key endogenous compounds such as thyroid/steroid hormones, as well as in the detoxification of xenobioticsincluding drugs. The present study constitutes part of an overall effort in establishing the zebrafish as a model for studying drug sulfation. By searching GenBank database, we have identified sequences encoding three new zebrafish SULTs. These three novel zebrafish SULTs, designated SULT3 ST4, SULT3 ST5 and SULT1 ST9, were cloned, expressed, purified, and characterized. SULT3 ST4 showed strong activity toward endogenous compound such as dehydroepiandrosterine (DHEA), pregnenolone, and 17β-estradiol. SULT3 ST5 showed weaker, but significant, activities toward endogenous compounds such as DHEA and corticosterone, and xenobiotics including mestranol, β-naphthylamine, β-naphthol, and butylated hydroxyl anisole (BHA). SULT1 ST9, on the other hand, appeared to be mostly involved in the metabolism and detoxification of xenobiotics such as β-naphthol, β-naphthylamine, caffeic acid and gallic acid. pH-dependency and kinetic studies were performed using these three enzymes with DHEA, β-naphthol, β-naphthylamine, and 17β-estradiol as substrates. RT-PCR was carried out to investigate the expression of these three novel zebrafish SULTs during various developmental stages from embryogenesis to maturity.
Ming-Cheh Liu, PhD (Committee Chair)
Ezdihar Hassoun, PhD (Committee Member)
Katherine Wall, PhD (Committee Member)
Pharmacology; Toxicology
Zebrafish; Sulfotransferases; SULTs; endogenous; xenobiotics; compunds

Recommended Citations

Citations

  • Mohammed, Y. I. (2011). Identification, Characterization, and Ontogenic Study of Three Novel Zebrafish Cytosolic Sulfotransferases (SULTs) [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1303486828

    APA Style (7th edition)

  • Mohammed, Yasir. Identification, Characterization, and Ontogenic Study of Three Novel Zebrafish Cytosolic Sulfotransferases (SULTs). 2011. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1303486828.

    MLA Style (8th edition)

  • Mohammed, Yasir. "Identification, Characterization, and Ontogenic Study of Three Novel Zebrafish Cytosolic Sulfotransferases (SULTs)." Master's thesis, University of Toledo, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=mco1303486828

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.