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The Na/K-ATPase/Caveolin-1 Interaction Regulates Cell Growth

Dong, Shuai
http://orcid.org/0000-0002-2434-2430
http://rave.ohiolink.edu/etdc/view?acc_num=mco1305223012

Abstract Details

2011, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Medicine.
The Na/K-ATPase α1 subunit contains a highly conserved caveolin-1 binding motif (CBM). We have previously shown that the interaction between the α1 CBM and caveolin-1 regulates the formation of caveolae, and intracellular trafficking and distribution of cholesterol. Here we demonstrate an important role of this interaction in control of cell growth. First, we find that knockdown of either the Na/K-ATPase or caveolin-1 inhibits cell proliferation and that this inhibition is greatly enhanced by modest depletion of cellular cholesterol. Second, the expression of wild-type α1, but not the CBM mutant, is capable of restoring normal growth in LLC-PK1 cells. Finally, we find that the expression of N-terminus of the α1 subunit inhibits the interaction between the Na/K-ATPase and caveolin-1. Consequently, it alters normal trafficking of caveolin-1 and cholesterol, and inhibits cell growth. Taken together, our new findings demonstrate that the highly conserved caveolin binding motif of the Na/K-ATPase plays an important role in regulation of cell growth by affecting the trafficking and distribution of caveolin-1 and cholesterol.
Zijian Xie, PhD (Committee Chair)
Lijun Liu, MD (Committee Member)
Jiang Liu, PhD (Committee Member)
61 p.
Biomedical Research; Pharmacology; Physiology
Na/K-ATPase; Caveolin-1; Cholesterol; Cell growth

Recommended Citations

Citations

  • Dong, S. (2011). The Na/K-ATPase/Caveolin-1 Interaction Regulates Cell Growth [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1305223012

    APA Style (7th edition)

  • Dong, Shuai. The Na/K-ATPase/Caveolin-1 Interaction Regulates Cell Growth. 2011. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1305223012.

    MLA Style (8th edition)

  • Dong, Shuai. "The Na/K-ATPase/Caveolin-1 Interaction Regulates Cell Growth." Master's thesis, University of Toledo, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=mco1305223012

    Chicago Manual of Style (17th edition)

mco1305223012
452
© 2011, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.