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mco1357227508.pdf (3.93 MB)
ETD Abstract Container
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Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation
Author Info
Lu, Ran
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=mco1357227508
Abstract Details
Year and Degree
2013, Doctor of Philosophy (PhD), University of Toledo, College of Medicine.
Abstract
Ultraviolet B (UVB) irradiation diminishes surface densities of Langerhans cells (LCs) in the epidermis of the skin. Other leukocytes, such as neutrophils and macrophages, are recruited to the irradiated sites due to chemo-attracting factors released from the altered microenvironment. However, there is little evidence directly demonstrating the behavior of LCs as well as other leukocytes in response to UVB radiation, and it is also unknown whether cellular interaction occurs during skin inflammation. In the present study, we developed a direct visualization system to observe the dynamic behaviors of both LCs and neutrophils responding to 3000J/m2 UVB in the skin. Using intravital confocal microscopy and genetically manipulated transgenic mice, we have found that neutrophils are recruited to inflammatory sites immediately after UVB irradiation. Skin-infiltrating neutrophils can interact with LCs upon activation and can last for several hours. Twenty-four hours after irradiation, LCs migrated to deeper skin layers, resulting in a 30% reduction of LC number in the epidermal compartment, whereas the number of recruited neutrophils increased over time. LCs completely disappeared from the epidermis 4 days after UVB treatment. Depletion of neutrophils by anti-Ly6G monoclonal antibody (mAb) administration did not affect LCs migration in response to the same UVB radiation, but rather promoted LC precursors’ repopulation to the epidermis after UVB treatment. In addition, depletion of neutrophils greatly diminished the magnitude of UVB-induced ear swelling, suggesting that neutrophils are crucial for UVB induced skin inflammation. Not only do our data unveil the behavior and cell-cell interaction of LCs and neutrophils upon UVB irradiation, they also indicate for the first time that neutrophils play an important role in LC repopulation.
Committee
Akira Takashima, MD,PhD (Committee Chair)
Kevin Pan, MD,PhD (Committee Member)
Mark Wooten, PhD (Committee Member)
Andrea Kalinoski, PhD (Committee Member)
Anthony Quinn, PhD (Committee Member)
Pages
99 p.
Subject Headings
Immunology
Keywords
Intravital imaging
;
UVB
;
Skin inflammation
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Citations
Lu, R. (2013).
Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation
[Doctoral dissertation, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1357227508
APA Style (7th edition)
Lu, Ran.
Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation.
2013. University of Toledo, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=mco1357227508.
MLA Style (8th edition)
Lu, Ran. "Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation." Doctoral dissertation, University of Toledo, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=mco1357227508
Chicago Manual of Style (17th edition)
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Document number:
mco1357227508
Download Count:
409
Copyright Info
© 2013, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.